PDF(Pubmed)
Abstract：
Regulation of microtubule dynamics is crucial during key developmental transitions such as gametogenesis, fertilization, embryogenesis, and seed formation, where cells undergo rapid changes in shape and function. In plants, katanin plays an essential role in microtubule dynamics. This study investigates two seed developmental mutants in Arabidopsis thaliana, named elk5-1D (erecta-like 5, ELK5) and loo1 (lollipop 1), which are characterized by round seeds, dwarfism, and fertility defects. Notably, elk5-1D exhibits a dominant inheritance pattern, whereas loo1 is recessive. Through positional cloning, we identified both mutants as new alleles of the KATANIN 1 (KTN1) gene, which encodes a microtubule-severing enzyme critical for cell division and morphology. Mutations in KTN1 disrupt embryo cell division and lead to the emergence of a twin embryo phenotype. Our findings underscore the essential role of KTN1 in fertility and early embryonic development, potentially influencing the fate of reproductive cells.
摘要：
微管动力学的调节在关键的发育转变过程中至关重要，例如配子发生，受精,胚胎发生,和种子形成，细胞在形状和功能上经历快速变化。在植物中,katanin在微管动力学中起着至关重要的作用。本研究调查了拟南芥中的两个种子发育突变体，命名为elk5-1D（类直立5，ELK5）和loo1（棒棒糖1），以圆形种子为特征，侏儒症,和生育缺陷。值得注意的是,elk5-1D表现出显性遗传模式，而loo1是隐性的。通过位置克隆，我们将这两个突变体鉴定为KATANIN1（KTN1）基因的新等位基因，它编码一种对细胞分裂和形态至关重要的微管切断酶。KTN1中的突变破坏胚胎细胞分裂并导致双胚胎表型的出现。我们的发现强调了KTN1在生育力和早期胚胎发育中的重要作用。可能影响生殖细胞的命运。
