背景:这项研究探讨了光生物调节疗法(PBMT)在增强正畸牙齿移动(OTM)中的有效性,成骨,和血管生成通过一系列全面的体外和体内研究。体外实验涉及共培养MC3T3-E1和HUVEC细胞以评估PBMT对细胞增殖的影响,成骨,血管生成,和相关的基因表达。同时,体内实验使用OTM大鼠模型,以特定的能量密度进行激光照射。
方法:体外实验涉及共培养用PBMT处理的MC3T3-E1和HUVEC细胞,能够全面评估细胞增殖,成骨,血管生成,和基因表达。在体内,将OTM大鼠模型以指定的能量密度进行激光照射。进行统计分析以评价观察到的差异的显著性。
结果:结果显示,与对照组相比,PBMT治疗组的血管形成和新骨生成显着增加。体外,PBMT显示对细胞增殖的积极作用,成骨,血管生成,和共培养模型中的基因表达。在体内,在比能量密度下的激光照射显着增强了OTM,血管生成,和成骨。
结论:这项研究强调了PBMT在改善正畸后骨骼质量方面的巨大潜力。观察到的血管生成和骨生成的增强表明PBMT在优化正畸实践中的治疗结果中的关键作用。该发现将PBMT定位为一种有前途的治疗干预措施,可以无缝地整合到正畸协议中。提供了一个新的维度来提高整体治疗效果。在实验室之外,这些结果表明PBMT在临床情景中的实际意义,强调其有助于推进正畸治疗的潜力。有必要在正畸实践中进一步探索PBMT,以释放其全部治疗潜力。
BACKGROUND: This
study explores the effectiveness of Photobiomodulation Therapy (PBMT) in enhancing orthodontic tooth movement (OTM), osteogenesis, and angiogenesis through a comprehensive series of in vitro and in vivo investigations. The in vitro experiments involved co-culturing MC3T3-E1 and HUVEC cells to assess PBMT\'s impact on cell proliferation, osteogenesis, angiogenesis, and associated gene expression. Simultaneously, an in vivo experiment utilized an OTM rat model subjected to laser irradiation at specific energy densities.
METHODS: In vitro experiments involved co-culturing MC3T3-E1 and HUVEC cells treated with PBMT, enabling a comprehensive assessment of cell proliferation, osteogenesis, angiogenesis, and gene expression. In vivo, an OTM rat model was subjected to laser irradiation at specified energy densities. Statistical analyses were performed to evaluate the significance of observed differences.
RESULTS: The results revealed a significant increase in blood vessel formation and new bone generation within the PBMT-treated group compared to the control group. In vitro, PBMT demonstrated positive effects on cell proliferation, osteogenesis, angiogenesis, and gene expression in the co-culture model. In vivo, laser irradiation at specific energy densities significantly enhanced OTM, angiogenesis, and osteogenesis.
CONCLUSIONS: This
study highlights the substantial potential of PBMT in improving post-orthodontic bone quality. The observed enhancements in angiogenesis and osteogenesis suggest a pivotal role for PBMT in optimizing treatment outcomes in orthodontic practices. The findings position PBMT as a promising therapeutic intervention that could be seamlessly integrated into orthodontic protocols, offering a novel dimension to enhance overall treatment efficacy. Beyond the laboratory, these results suggest practical significance for PBMT in clinical scenarios, emphasizing its potential to contribute to the advancement of orthodontic treatments. Further exploration of PBMT in orthodontic practices is warranted to unlock its full therapeutic potential.