目的:本研究评估了西维美林和不同浓度的阿拉伯树胶对口干症诱导甲氨蝶呤大鼠腮腺的影响。
方法:将一百二十五只大鼠分为五个相等的组,每组二十五只。第一组的大鼠接受基本饮食,而第二组的人,III,IV,并且V在第一天接受20mg/kgMTX作为单次腹膜内剂量。第三组口服10mg/kgCVM,每天溶于生理盐水,其他两组接受10%W/V的GA水悬浮液。因此,IV组每天口服2ml/kg混悬液,而第V组每天口服3ml/kg混悬液。9天后,对腮腺进行仔细解剖,并准备进行常规组织学染色的苏木精和曙红(H&E)染色以及caspase-3和Ki67免疫组织化学染色.使用单向ANOVA和Tukey多重比较事后检验对来自α-Caspase-3染色和Ki67染色的定量数据进行统计学分析。
结果:关于caspase-3和Ki67免疫组织化学染色,单因素方差分析显示,5组之间存在显著差异.对于Caspase-3,最高的平均值是II组(54.21±6.90),最低的平均值是I组(15.75±3.67)。其他三组Ⅲ组的平均值分别为31.09±5.90,30.76±5.82,20.65±3.47,IV,V,分别。对于Ki67,最高的平均值是I组(61.70±6.58),最低值为II组(18.14a±5.16)。其他三组III组的平均值分别为34.4±9.27、48.03±8.40和50.63±8.27,IV,V,分别。
结论:GA,而不是通常使用的药物CVM,对口干症患者的唾液腺有理想的影响。
OBJECTIVE: This
study assessed the effect of cevimeline and different concentrations of gum arabic on the parotid gland of rats being given xerostomia-inducing methotrexate.
METHODS: One hundred twenty-five rats were divided into five equal groups of twenty-five each. The rats in Group I received basic diets, while those in Groups II, III, IV, and V received 20 mg/kg MTX as a single intraperitoneal dose on day one. Group III received 10 mg/kg CVM dissolved in saline orally and daily, and the other two groups received a 10% W/V aqueous suspension of GA. Therefore, Group IV received 2 ml/kg suspension orally and daily, while Group V received 3 ml/kg suspension orally and daily. After 9 days, the parotid glands were dissected carefully and prepared for hematoxylin and eosin (H&E) staining as a routine histological stain and caspase-3 and Ki67 immunohistochemical staining. Quantitative data from α-Caspase-3 staining and Ki67 staining were statistically analysed using one-way ANOVA followed by Tukey\'s multiple comparisons post hoc test.
RESULTS: Regarding caspase-3 and Ki67 immunohistochemical staining, one-way ANOVA revealed a significant difference among the five groups. For Caspase-3, the highest mean value was for group II (54.21 ± 6.90), and the lowest mean value was for group I (15.75 ± 3.67). The other three groups had mean values of 31.09 ± 5.90, 30.76 ± 5.82, and 20.65 ± 3.47 for groups III, IV, and V, respectively. For Ki67, the highest mean value was for group I (61.70 ± 6.58), and the lowest value was for group II (18.14a ± 5.16). The other three groups had mean values of 34.4 ± 9.27, 48.03 ± 8.40, and 50.63 ± 8.27 for groups III, IV, and V, respectively.
CONCLUSIONS: GA, rather than the normally used drug CVM, had a desirable effect on the salivary glands of patients with xerostomia.