■器官缺血再灌注(IR)是与创伤手术等各种情况相关的常见临床状况,器官移植,和心肌缺血。目前治疗IR损伤的方法有局限性,和纳米技术,特别是氧化锌纳米颗粒(ZnONPs),为疾病诊断和治疗提供了新的途径。在这项研究中,我们研究了ZnONPs对大鼠肝脏缺血再灌注(IR)损伤的保护作用和抗凋亡作用。
■将48只雄性大鼠分为6组:假,ZnO5,ZnO10,缺血再灌注(IR),IR+ZnO5,和IR+ZnO10。通过肝酶(AST,ALT,胆红素,ALP),生物化学(TAC,TNF-α,和MDA),分子检查(Bcl2,BAX),和组织病理学评估(H&E,TUNEL)。
■用ZnO5和ZnO10预处理可改善IR肝损伤中的肝功能,降低氧化剂(P=0.03)和炎症介质的水平,细胞凋亡减少(P=0)。发现ZnO10对缺血再灌注损伤的影响比ZnO5更大。组织病理学检查还显示,IRZnO5和IRZnO10组的变化呈剂量依赖性降低。
■施用ZnO5和ZnO10改善IR后的肝功能。这项研究的发现表明,ZnONPs对大鼠肝脏缺血再灌注损伤的氧化应激和凋亡具有保护作用。这些结果可能对开发缺血再灌注治疗的先进方法具有重要意义。
UNASSIGNED: Organ ischemia-reperfusion (IR) is a common clinical condition associated with various situations such as trauma surgery, organ transplantation, and myocardial ischemia. Current therapeutic methods for IR injury have limitations, and nanotechnology, particularly zinc oxide nanoparticles (ZnO NPs), offers new approaches for disease diagnosis and treatment. In this study, we investigated the protective and anti-apoptotic effects of ZnO NPs in liver ischemia-reperfusion (IR) injury in rats.
UNASSIGNED: Forty-eight male rats were divided into six groups: sham, ZnO5, ZnO10, ischemia-reperfusion (IR), IR+ZnO5, and IR+ZnO10. The protective effect of ZnO NPs was evaluated by liver enzymes (AST, ALT, Bilirubin, ALP), biochemical (TAC, TNF-α, and MDA), molecular examinations (Bcl2, BAX), and histopathological evaluations (H&E, TUNEL).
UNASSIGNED: Pre-treatment with ZnO5 and ZnO10 improved hepatic function in IR liver injury, attenuated the levels of oxidants (P = 0.03) and inflammatory mediators, and reduced apoptosis (P = 0). ZnO10 was found to have a greater effect on ischemic reperfusion injury than ZnO5 did. Histopathological examination also showed a dose-dependent decrease in alterations in the IR+ZnO5 and IR+ZnO10 groups.
UNASSIGNED: Administration of ZnO5 and ZnO10 improved liver function after IR. The findings of this study suggest that ZnO NPs have a protective effect against oxidative stress and apoptosis in liver ischemia-reperfusion injury in rats. These results may have important implications for developing advanced methods in ischemia-reperfusion treatment.