PDF(Pubmed)
Abstract：
Nowadays, the extremely-low-frequency electromagnetic field (ELF-EMF) is recognized as environmental pollution. The data indicate that the ELF-EMF may affect factors related to epigenetic regulation and alter important biological processes in the uterus. The impact of the ELF-EMF on apoptosis and oxidative-stress-related genes has not been documented in porcine endometrium. This raises the question of whether the exposure to the ELF-EMF can induce apoptosis and/or oxidative stress in the endometrium of pigs during the peri-implantation period. Porcine endometrial slices (100 ± 5 mg) collected (n = 5) during the peri-implantation period were treated in vitro with ELF-EMF at a frequency of 50 Hz and flux density of 8 × 104 mG for 2 h. To determine the effect of ELF-EMF on apoptosis and oxidative stress in the endometrium, CASP3, CASP7, CIDEB, GADD45G, NOS1, NOS2, NOS3, and TP53I3 mRNA transcript were analyzed using real-time PCR, and protein abundance of CASP3, CASP7 using Western blot, and eNOS using ELISA were determined. Moreover, CASP3/7 and NOS activity was analyzed using flow cytometry and colorimetry, respectively. The decreased CASP7 and increased NOS3 mRNA transcript and protein abundance in ELF-EMF-treated endometrium were observed. Moreover, CIDEB, GADD45G, and TP53I3 mRNA transcript abundance was increased. Only p ≤ 0.05 was considered a statistically significant difference. The documented alterations indicate the potential of the ELF-EMF to affect apoptosis and generate oxidative stress in the endometrium. The insight into observed consequences documents for the first time the fact that the ELF-EMF may influence endometrial cell proliferation, angiogenesis, and/or tissue receptivity during peri-implantation.
摘要：
如今,极低频电磁场(ELF-EMF)被认为是环境污染。数据表明，ELF-EMF可能会影响与表观遗传调控相关的因素，并改变子宫中的重要生物学过程。ELF-EMF对细胞凋亡和氧化应激相关基因的影响尚未在猪子宫内膜中得到证实。这提出了一个问题，即在围植入期暴露于ELF-EMF是否可以诱导猪子宫内膜的细胞凋亡和/或氧化应激。在围植入期收集的猪子宫内膜切片（100±5mg）（n=5），用ELF-EMF以50Hz的频率和8×104mG的通量密度在体外处理2h。为了确定ELF-EMF对子宫内膜细胞凋亡和氧化应激的影响，CASP3,CASP7,CIDEB,GADD45G,使用实时PCR分析NOS1，NOS2，NOS3和TP53I3mRNA转录本，和蛋白质丰度的CASP3，CASP7使用蛋白质印迹，和eNOS使用ELISA进行测定。此外，使用流式细胞术和比色法分析CASP3/7和NOS活性，分别。在ELF-EMF处理的子宫内膜中观察到CASP7降低和NOS3mRNA转录物和蛋白质丰度增加。此外，CIDEB,GADD45G,TP53I3mRNA转录物丰度增加。只有p≤0.05被认为是统计学上显著的差异。记录的改变表明ELF-EMF可能影响子宫内膜中的细胞凋亡并产生氧化应激。对观察到的后果的洞察首次记录了ELF-EMF可能影响子宫内膜细胞增殖的事实，血管生成,和/或在围植入期间的组织接受性。
