本试验旨在研究不同土壤条件下施用丛枝菌根真菌(AMF)对玉米根系生长及产量形成的影响。本研究是在沙质土壤(S)和盐碱土(Y)下进行的,应用AMF处理(AM)和不应用AMF处理(CK)。根的特征,产量,并对玉米品质进行了测定。采用高通量测序技术评价AMF对土壤微生物群落结构的影响,阐明了土壤微生物与土壤理化性质的相关性。结果表明,在沙土和盐碱土条件下,施用AMF显著促进了玉米根系生长,产量,粮食质量,和土壤有效氮(AN),有效磷(AP),与CK处理相比,有效钾(AK)含量。土壤微生物α多样性分析表明,施用AMF能有效提高土壤微生物的多样性和丰富度。主坐标分析(PCoA)和微生物群落结构分析显示,沙质土壤(SAM)的AM处理和沙质土壤(SCK)的CK处理之间的细菌群落存在显着差异。盐碱土(YAM)的AM处理和盐碱土(YCK)的CK处理之间的细菌和真菌群落均存在显着差异。此外,发现微生物群落与土壤理化性质之间存在显着相关性,比如AN,AP,AK,土壤盐分(SS),和有机质(OM)含量。AMF应用对细菌群落的影响大于对真菌群落的影响。这项研究表明,使用AMF作为生物真菌肥料可以有效地提高春玉米的产量。特别是在沙土和盐渍土的产量增加和质量稳定性方面,从而促进安全和可持续的种植做法。
This study aimed to investigate the effects of applying arbuscular mycorrhizal fungi (AMF) on maize root growth and yield formation under different soil conditions. This study was conducted under sandy soil (S) and saline-alkali soil (Y), with treatments of AMF application (AM) and no AMF application (CK). The root characteristics, yield, and quality of maize were measured. High-throughput sequencing technology was employed to assess the impact of AMF on the soil microbial community structure, and the correlation between soil microbes and soil physicochemical properties was elucidated. The results show that under both sandy and saline-alkali soil conditions, AMF application significantly enhanced maize root growth, yield, grain quality, and soil available nitrogen (AN), available phosphorus (AP), and available potassium (AK) contents compared to the CK treatment. Soil microbial Alpha diversity analysis indicated that AMF application effectively increased soil microbial diversity and richness. Principal coordinate analysis (PCoA) and microbial community structure analysis revealed significant differences in bacterial communities between AM treatment in sandy soil (SAM) and CK in sandy soil (SCK), and significant differences in both bacterial and fungal communities between AM treatment in saline-alkali soil (YAM) and CK in saline-alkali soil (YCK). Furthermore, significant correlations between microbial communities and soil physicochemical properties were found, such as AN, AP, AK, soil salinity (SS), and organic matter (OM) content. AMF application had a greater impact on bacterial communities than on fungal communities. This study demonstrated that the use of AMF as a bio-fungal fertilizer was effective in improving spring maize yields, especially in terms of yield increase and quality stability in sandy and saline soils, thereby contributing to safe and sustainable cropping practices.