背景:肝纤维化是全世界的主要健康问题,没有有效的治疗方法可以治愈。因此,本研究旨在评估芹菜素对CCl4诱导的小鼠肝纤维化的抗纤维化功效.
方法:将48只小鼠分为6组。G1:正常控制,G2:CCl4控制,G3:水飞蓟素(100mg/kg),G4和G5:芹菜素(2和20mg/Kg),G6:单独的芹菜素(20mg/Kg)。第2、3、4和5组给予CCl4(0.5mL/kg。i.p.)每周两次,共六周。AST的水平,ALT,TC,TG,和血清中的TB和IL-1β,评估组织匀浆中的IL-6和TNF-α。还进行了通过H&E染色和肝组织免疫染色的组织学研究。
结果:CCl4攻击组显示血清AST升高(4倍),ALT(6倍),和TB(5倍)。水飞蓟素和芹菜素治疗显着改善这些肝脏生物标志物。CCl4攻击组显示CAT水平降低(89%),GSH(53%),MDA增加(3倍)。水飞蓟素和芹菜素处理均显着改变了组织匀浆中的这些氧化标志物。CCl4治疗组显示IL-1β增加两倍,IL-6和TNF-α水平。水飞蓟素和芹菜素处理显著降低IL-1β,IL-6和TNF-α水平。芹菜素治疗抑制血管生成活性,VEGF(血管内皮生长因子)在肝组织中的表达减少,血管内皮细胞抗原表达(CD34)下降。
结论:最后,这些数据共同暗示芹菜素可能具有抗纤维化特性,这可以用它的抗炎作用来解释,抗氧化剂,和抗血管生成活性。
BACKGROUND: Hepatic fibrosis is a major health problem all over the world, and there is no effective treatment to cure it. Hence, the current
study sought to assess the anti-fibrotic efficacy of
apigenin against CCl4-induced hepatic fibrosis in mice.
METHODS: Forty-eight mice were put into six groups. G1: Normal Control, G2: CCl4 Control, G3: Silymarin (100 mg/kg), G4 and G5:
Apigenin (2 &20 mg/Kg), G6:
Apigenin alone (20 mg/Kg). Groups 2, 3, 4, and 5 were given CCl4 (0.5 mL/kg. i.p.) twice/week for six weeks. The level of AST, ALT, TC, TG, and TB in serum and IL-1β, IL-6, and TNF-α in tissue homogenates were assessed. Histological studies by H&E staining and Immunostaining of liver tissues were also performed.
RESULTS: The CCl4-challenged group showed increased serum AST (4-fold), ALT (6-fold), and TB (5-fold). Both silymarin and
apigenin treatments significantly improved these hepatic biomarkers. The CCl4-challenged group showed reduced levels of CAT (89%), GSH (53%), and increased MDA (3-fold). Both silymarin and apigenin treatments significantly altered these oxidative markers in tissue homogenates. The CCl4-treated group showed a two-fold increase in IL-1β, IL-6, and TNF-α levels. Silymarin and
apigenin treatment considerably decreased the IL-1β, IL-6, and TNF-α levels. Apigenin treatment inhibited angiogenic activity, as evidenced by a decrease in VEGF (vascular endothelial growth factor) expression in liver tissues, and a decline in vascular endothelial cell antigen expression (CD34).
CONCLUSIONS: Finally, these data collectively imply that apigenin may have antifibrotic properties, which may be explained by its anti-inflammatory, antioxidant, and antiangiogenic activities.