糖尿病并发症,包括周围神经病变,白内障,伤口愈合受损,血管损伤,动脉壁硬化和视网膜病变疾病,是当今世界人口面临的最主要的健康问题之一。筛选了22种秘鲁植物提取物对大鼠晶状体醛糖还原酶(RLAR)和DPPH自由基清除的潜在抑制活性。其中,我们发现TanacetumpartheniumL.(TP)具有RLAR,AGEs和DPPH自由基清除活性。我们首次通过超滤(UF)和DPPH筛选TP中针对RLAR和DPPH的活性成分。通过Sephadex柱层析分离TP中的化合物,并通过MS和NMR光谱分析确定其结构。在分离的化合物中,阿魏酸,芹菜素,木犀草素-7-O-葡萄糖苷,木犀草素,chrysosplool,和山奈酚对RLAR和DPPH自由基清除显示出有效的抑制作用,IC50值为1.11-3.20和6.44-16.23μM。此外,这些化合物抑制了山梨糖醇在大鼠晶状体和阿魏酸中的积累,木犀草素-7-O-葡萄糖苷,和木犀草素具有AGEs抑制活性,IC50值为3.43-6.73μM。总之,我们的研究为进一步研究秘鲁植物糖尿病并发症的治疗和预防提供了有趣的植物,可以为传统用途提供科学依据。
Diabetes complications, including peripheral neuropathy, cataracts, impaired wound healing, vascular damage, arterial wall stiffening and retinopathy diseases, are among the most predominant health problems facing the world\'s population today. The 22 Peruvian plant extracts were screened for their potential inhibitory activity against rat lens aldose reductase (RLAR) and DPPH radical scavenging. Among them, we have found that Tanacetum parthenium L. (TP) has the RLAR, AGEs and DPPH radical scavenging activities. We used for screening of active components in TP against RLAR and DPPH for the first time by ultrafiltration (UF) and DPPH. Compounds in TP were isolated by Sephadex column chromatography and their structures were established by MS and NMR spectroscopic analyses. Among the isolated compounds, ferulic acid,
apigenin, luteolin-7-O-glucoside, luteolin, chrysosplenol, and kaempferol showed potent inhibition with IC50 values of 1.11-3.20 and 6.44-16.23 μM for RLAR and DPPH radical scavenging. Furthermore, these compounds suppressed sorbitol accumulation in rat lenses and ferulic acid, luteolin-7-O-glucoside, and luteolin have AGEs inhibitory activities with IC50 values of 3.43-6.73 μM. In summary, our study provides interesting plants for further study with respect to the treatment and prevention of diabetic complication of Peruvian plant and can provide the scientific base of the traditional uses.