air quality

空气质量
  • 文章类型: Case Reports
    野火烟雾(WFS)是对全球健康的紧迫和快速增长的威胁。除了对肺功能的明显威胁,在WF季节期间,心脏异常或心肌梗塞的增加已被记录,但对WFS对心血管健康的影响知之甚少。我们通过一系列年轻的病例,调查了非职业性WFS暴露对休息时和分级握力运动中心血管和肺功能的影响,健康成年人(n=4,25±6岁)在空气质量差或好≥3天后进行评估。外周和估计的中枢血压,血管僵硬度,和微血管功能(近红外光谱,NIRS)在休息时进行评估,在有节奏的手握练习中。WFS似乎没有改变静息的外周,中央BP,或血管僵硬(所有,p>0.05)。NIRS-血管闭塞试验(NIRS-VOT)的斜率1和斜率2在条件之间没有差异(p>0.05)。运动期间SmO2的变化较低(p=0.02,ηp2${\\eta}_{\\mathrm{p}}^2$$=0.62),空气质量较差。这些初步发现表明,在健康成年人的运动过程中,环境WFS暴露对肌肉微血管功能的影响不大。未来的工作需要阐明WFS暴露的生理变化和心血管事件风险的增加。也许是通过体力活动加剧的。
    Wildfire smoke (WFS) is an urgent and rapidly growing threat to global health. Aside from obvious threats to pulmonary function, increases in cardiac abnormalities or myocardial infarction have been documented during WF season, but little is known about the effects of WFS on cardiovascular health. We investigated the effect of nonoccupational WFS exposure on cardiovascular and pulmonary function at rest and during graded handgrip exercise through a case series of young, healthy adults (n = 4, 25 ± 6 years) assessed after ≥3 days of bad or good air quality. Peripheral and estimated central blood pressures, vascular stiffness, and microvascular function (Near infrared spectroscopy, NIRS) were assessed at rest, and during rhythmic handgrip exercise. WFS did not appear to alter resting peripheral, central BP, or vascular stiffness (all, p > 0.05). Slope 1 and slope 2 from the NIRS-vascular occlusion test (NIRS-VOT) were not different between conditions (p > 0.05). The change in SmO2 during exercise was lower (p = 0.02, η p 2 $$ {\\eta}_{\\mathrm{p}}^2 $$  = 0.62) with bad air quality. These preliminary findings suggest modest effects of environmental WFS exposure on muscle microvascular function during exercise in healthy adults. Future work is needed to elucidate the physiological changes with WFS exposure and the increased risk of cardiovascular events, perhaps exacerbated through physical activity.
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  • 文章类型: Journal Article
    空气污染是城市地区可持续发展面临的重要问题。反对印度的快速城市化,一些城市地区表现出人口增长和发达地区扩张的并存以及广泛的空间异质性。城市地区的增长和城市扩张之间的相互作用机制对于空气污染的治理具有极其重要的意义。从今以后,本调查利用地理加权回归(GWR)来检查城市扩张和人口增长对空气质量的影响。这项研究将使用十年来的数据来分析印度8个大都市从2010年到2020年的PM2.5水平变化。研究结果表明,城市增长动态和空气污染水平之间存在空间异质性。城市增长和城镇扩张对空气质量表现出显著的积极影响,尽管在不断扩大的城市地区内,填充的增长会显著影响空气质量。鉴于发展中国家城市发展的独特轨迹,这项研究为城市管理者促进城市可持续发展提供了许多建议。
    Air pollution is a matter of great significance that confronts the sustainable progress of urban areas. Against India\'s swift urbanization, several urban areas exhibit the coexistence of escalating populace and expansion in developed regions alongside extensive spatial heterogeneity. The interaction mechanism between the growth of urban areas and the expansion of cities holds immense importance for the remediation of air pollution. Henceforth, the present investigation utilizes geographically weighted regression (GWR) to examine the influence of urban expansion and population growth on air quality. The examination will use a decade of data on the variation in PM2.5 levels from 2010 to 2020 in eight Indian metropolitan cities. The study\'s findings demonstrate a spatial heterogeneity between urban growth dynamics and air pollution levels. Urban growth and the expansion of cities demonstrate notable positive impacts on air quality, although the growth of infilling within expanding urban areas can significantly affect air quality. Given the unique trajectories of urban development in developing countries, this research provides many suggestions for urban administrators to foster sustainable urban growth.
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  • 文章类型: Journal Article
    建筑物的通风对于确保室内健康至关重要,特别是当要求在室内进行体育活动时,大流行强调了开发新的管理方法以确保足够的通风的必要性。在西班牙,没有具体的通风规定来防止冠状病毒等病原体的传播。因此,有必要有一个理论工具来计算占用率,以保持体育设施在最佳安全条件下。拟议的理论方法基于对欧洲标准化文件中数学表达式的分析,并将CO2的浓度用作生物流出物。它还基于背景和临界浓度的概念,这使得它的应用可以外推到病原体引起的未来危机。这项研究为体育中心提供了一个独特而新颖的数据集。为此,计算方法应用于莫斯托莱斯市议会提供的数据集,西班牙,在COVID-19发病率最高的大流行年份,当时政府将COVID-19病假与职业事故同化。市议会提供的此类病假数据对应于2020年3月至2022年2月之间的时期。同样,按活动划分的体育设施平均使用数据,由体育部提供,对应于2020年和2021年。这样,有可能验证预防任何类型冠状病毒传播的有效性。总之,实施基于二氧化碳作为生物流出物的浓度的理论占用率计算方法可以成为管理由空气中的病原体或危险化学品引起的未来危机的有效工具,并在体育馆等体育中心展示了其有效性,运动场,和COVID-19大流行期间的室内游泳池。
    The ventilation of buildings is crucial to ensure indoor health, especially when demanding physical activities are carried out indoors, and the pandemic has highlighted the need to develop new management methods to ensure adequate ventilation. In Spain, there are no specific ventilation regulations to prevent the spread of pathogens such as the coronavirus. Therefore, it is necessary to have a theoretical tool for calculating occupancy to maintain sports facilities in optimal safety conditions. The proposed theoretical method is based on the analysis of mathematical expressions from European standardisation documents and uses the concentration of CO2 as a bioeffluent. It is also based on the concept of background and critical concentration, which allows its application to be extrapolated to future crises caused by pathogens. This study presents a unique and novel dataset for sports centres. For this purpose, the calculation methods were applied to the data set provided by Mostoles City Council, Spain, during the pandemic years with the highest incidence of COVID-19, when the government introduced the assimilation of COVID-19 sick leave to occupational accidents. The data on this type of sick leave provided by the City Council correspond to the period between March 2020 and February 2022. Similarly, the data on the average use of sports facilities by activity, provided by the Sports Department, correspond to the years 2020 and 2021. In this way, it was possible to verify the effectiveness in preventing the spread of any type of coronavirus. In conclusion, the implementation of a theoretical occupancy calculation method based on the concentration of carbon dioxide as a bioeffluent can be an effective tool for the management of future crises caused by pathogens or hazardous chemicals in the air, and demonstrated its effectiveness in sports centres such as gyms, sports fields, and indoor swimming pools during the COVID-19 pandemic.
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  • 文章类型: Journal Article
    野火是对人类健康的重大威胁,部分原因是空气质量下降。规定的燃烧可以降低野火的严重程度,但也可能导致空气污染的增加。火灾和大气过程的复杂性导致在预测火灾对空气质量的影响时存在不确定性,并难以全面评估规定火灾扩大的成本和收益。通过对排放差异进行建模,表面条件,以及野火和规定烧伤之间的气象学,我们提出了在特定情况下这些火灾类型对空气质量影响的新颖比较。考虑了一次野火和两种规定的烧伤情况,针对潜在的烟雾暴露优化了一个规定的燃烧场景。我们发现PM2.5的排放量减少了52%,从0.27到0.14Tg,当火灾在规定的燃烧条件下燃烧时,显著降低PM2.5浓度。PM2.5暴露导致的短期死亡率过高是野火条件下40人死亡,默认和优化规定的燃烧情景下39人和15人死亡。分别。我们的发现表明处方烧伤,特别是当计划在尽量减少烟雾暴露的条件下,对于野火对空气质量和健康的影响可能是一个净好处。
    Wildfires are a significant threat to human health, in part through degraded air quality. Prescribed burning can reduce wildfire severity but can also lead to an increase in air pollution. The complexities of fires and atmospheric processes lead to uncertainties when predicting the air quality impacts of fire and make it difficult to fully assess the costs and benefits of an expansion of prescribed fire. By modeling differences in emissions, surface conditions, and meteorology between wildfire and prescribed burns, we present a novel comparison of the air quality impacts of these fire types under specific scenarios. One wildfire and two prescribed burn scenarios were considered, with one prescribed burn scenario optimized for potential smoke exposure. We found that PM2.5 emissions were reduced by 52%, from 0.27 to 0.14 Tg, when fires burned under prescribed burn conditions, considerably reducing PM2.5 concentrations. Excess short-term mortality from PM2.5 exposure was 40 deaths for fires under wildfire conditions and 39 and 15 deaths for fires under the default and optimized prescribed burn scenarios, respectively. Our findings suggest prescribed burns, particularly when planned during conditions that minimize smoke exposure, could be a net benefit for the impacts of wildfires on air quality and health.
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  • 文章类型: Journal Article
    在发展中经济体的城市中心,在不科学的管理的垃圾填埋场中发生废物火灾已成为一个紧迫的环境问题。在目前的工作中,进行了一项调查,以评估在高知的Brahmapuram城市固体废物处理厂(BMSWTP)发生的三起主要火灾事件对空气质量的影响,印度。最初,进行了基于Landsat的表面温度监测,以识别垃圾填埋场内的热点。对废物火灾期间不同污染物的排放进行了量化,并在基于卫星的非原位方法和基于现场的原位方法之间进行了比较。使用混合单颗粒拉格朗日综合轨迹(HYSPLIT)颗粒分散模型可视化了火灾期间释放的PM2.5颗粒的分散模式。填埋气体排放模型(LandGEM)用于量化废物存储过程中释放的温室气体(GHG)。然后将其与废物火灾期间的温室气体排放量进行比较。原位排放估算表明,BMSWTP的废物燃烧导致释放909.3MT的PM10,938.8MT的PM2.5,5832.9MT的CO,43.6公吨SOx,284.2MT的NOx,138,941.9MT的CO2,426.8MT的CH4和2665.1MT的VOC。然而,在原位和非原位发射估计之间观察到明显的差异,其中后者低估了实际排放量。大多数排放的PM2.5颗粒在盛行风的影响下向海洋传播,覆盖高知市政公司人口稠密的地区。废物火灾期间CH4和CO2的排放量与159天的CH4废物储存和51.8年的CO2废物储存的排放量相当,累积的全球变暖潜势为147.9GgCO2-e。
    The occurrence of waste fires in unscientifically managed landfill sites has become a pressing environmental issue in the urban centers of developing economies. In the present work, an investigation was carried out to evaluate the air quality implications of three major fire events that occurred at the Brahmapuram Municipal Solid Waste Treatment Plant (BMSWTP) in Kochi, India. Initially, Landsat-based surface temperature monitoring was conducted to identify the thermal hotspots within the landfill. The emissions of different pollutants during waste fires were quantified and compared between satellite-based ex-situ and field-based in-situ methods. The dispersion patterns of PM2.5 particles released during the fires were visualised using the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) particle dispersion model. The Landfill Gas Emissions Model (LandGEM) was employed to quantify the greenhouse gases (GHGs) released during waste storage, which was then compared with the GHGs emissions during waste fires. In-situ emission estimates showed that the combustion of waste at BMSWTP led to the release of 909.3 MT of PM10, 938.8 MT of PM2.5, 5832.9 MT of CO, 43.6 MT of SOx, 284.2 MT of NOx, 138,941.9 MT of CO2, 426.8 MT of CH4, and 2665.1 MT of VOC. However, a noticeable disparity was observed between the in-situ and ex-situ emission estimates, wherein the latter underestimated the actual emissions. Most of the emitted PM2.5 particles propagated oceanward under the influence of prevailing winds, covering the densely populated areas of Kochi municipal corporation. The amount of CH4 and CO2 emitted during the waste fires was on par with the emissions from 159 days of waste storage for CH4 and 51.8 years of waste storage for CO2, with a cumulative global warming potential of 147.9 Gg CO2-e.
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  • 文章类型: Journal Article
    自二十一世纪初以来,中国经济高速或中速增长,空气污染变得越来越严重。研究采用1998年至2019年遥感观测数据,采用标准差椭圆模型和空间自相关分析,探讨河南省PM2.5的时空分布特征。此外,应用多尺度地理加权回归模型(MGWR)来探索12个驱动因素的影响(例如,平均地表温度和二氧化碳排放量)对PM2.5浓度的影响。研究表明,(1)在22年的时间里,河南省的PM2.5年平均浓度表现出类似于字母“M”形状的趋势,河南省的总体趋势表明,PM2.5浓度的空间重心向北移动。(2)河南省PM2.5存在明显的空间聚集型,随着北部地区显示出空间热点的主要集中,而西部和南部地区主要是寒冷地区。(3)MGWR比GWR更有效地揭示了各尺度影响因素的空间异质性,从而使其成为研究PM2.5浓度背后驱动机制的更合适方法。(4)MGWR模型结果表明,各因素对PM2.5浓度的影响存在不同程度的空间异质性。总结上述结论,河南省大气环境管理还有很长的路要走,有关政策的制定要因地制宜,考虑不同影响因素对PM2.5影响的空间尺度效应。
    Since the start of the twenty-first century, China\'s economy has grown at a high or moderate rate, and air pollution has become increasingly severe. The study was conducted using data from remote sensing observations between 1998 and 2019, employing the standard deviation ellipse model and spatial autocorrelation analysis, to explore the spatiotemporal distribution characteristics of PM2.5 in Henan Province. Additionally, a multiscale geographically weighted regression model (MGWR) was applied to explore the impact of 12 driving factors (e.g., mean surface temperature and CO2 emissions) on PM2.5 concentration. The research revealed that (1) Over a period of 22 years, the yearly mean PM2.5 concentrations in Henan Province demonstrated a trend resembling the shape of the letter \"M\", and the general trend observed in Henan Province demonstrated that the spatial center of gravity of PM2.5 concentrations shifted toward the north. (2) Distinct spatial clustering patterns of PM2.5 were observed in Henan Province, with the northern region showing a primary concentration of spatial hot spots, while the western and southern areas were predominantly characterized as cold spots. (3) MGWR is more effective than GWR in unveiling the spatial heterogeneity of influencing factors at various scales, thereby making it a more appropriate approach for investigating the driving mechanisms behind PM2.5 concentration. (4) The results acquired from the MGWR model indicate that there are varying degrees of spatial heterogeneity in the effects of various factors on PM2.5 concentration. To summarize the above conclusions, the management of the atmospheric environment in Henan Province still has a long way to go, and the formulation of relevant policies should be adapted to local conditions, taking into account the spatial scale effect of the impact of different influencing factors on PM2.5.
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  • 文章类型: Journal Article
    城市温室气体排放监测对于评估减缓气候变化行动的影响至关重要。使用大气连续测量空气质量和二氧化碳(CO2),我们开发了一个梯度下降优化系统来估算巴黎市的排放量。我们评估了我们在第一个SARS-CoV-2相关封锁期间的联合CO2-CO-NOx优化,导致NOx排放量减少40%,CO2排放量减少30%,这与使用自下而上的活动数据进行的初步估算一致,但低于贝叶斯大气反演的减少估算值(50%)。在评估模型之前,我们首先对三个排放数据集进行深入分析。在巴黎周围地区(称为法兰西岛),由于所有数据集都受到报告的国家和地区总数的限制,因此在总数上达成了普遍协议。然而,数据集显示其部门分布以及种间比率存在分歧。季节性还显示与非工业固定燃烧(住宅和三次燃烧)相关的排放产品之间存在分歧。本文提供的结果表明,通过部署并置的大气温室气体和空气质量监测站,多物种方法有可能提供部门信息来监测城市地区的CO2排放。
    Urban greenhouse gas emissions monitoring is essential to assessing the impact of climate mitigation actions. Using atmospheric continuous measurements of air quality and carbon dioxide (CO2), we developed a gradient-descent optimization system to estimate emissions of the city of Paris. We evaluated our joint CO2-CO-NOx optimization over the first SARS-CoV-2 related lockdown period, resulting in a decrease in emissions by 40% for NOx and 30% for CO2, in agreement with preliminary estimates using bottom-up activity data yet lower than the decrease estimates from Bayesian atmospheric inversions (50%). Before evaluating the model, we first provide an in-depth analysis of three emission data sets. A general agreement in the totals is observed over the region surrounding Paris (known as Île-de-France) since all the data sets are constrained by the reported national and regional totals. However, the data sets show disagreements in their sector distributions as well as in the interspecies ratios. The seasonality also shows disagreements among emission products related to nonindustrial stationary combustion (residential and tertiary combustion). The results presented in this paper show that a multispecies approach has the potential to provide sectoral information to monitor CO2 emissions over urban areas enabled by the deployment of collocated atmospheric greenhouse gases and air quality monitoring stations.
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  • 文章类型: Journal Article
    空气污染是心血管疾病(CVD)的主要危险因素。迄今为止,有限的研究使用高分辨率暴露评估来估计环境空气污染对CVD死亡率的影响,这可能无法捕捉到曝光的空间变化,并在结果中引入偏差。此外,三年行动计划(TYAP,2018-2020)发布;因此,空气污染物的构成和健康影响可能已经改变。在这项研究中,我们以0.05°×0.05°分辨率估计了暴露于参数小于2.5µm的颗粒物(PM2.5)和臭氧(O3)对CVD死亡率的短期影响,并测量了TYAP在这些关联中的影响.我们使用具有空间权重矩阵的随机森林模型来获得高分辨率污染物浓度,并使用条件泊松回归来评估空气污染与心血管死亡率之间的关系。随着2018-2021年四川盆地(SCB)PM2.5和O3的增加10µg/m3,CVD死亡率增加1.0134(95%CI1.0102,1.0166)和1.0083(95%CI1.0060,1.0107),分别,使用高分辨率的空气污染物浓度,使用来自空气质量监测站(AQM)的数据,与1.0070(95%CI1.0052,1.0087)和1.0057(95%CI1.0037,1.0078)进行比较。TYAP之后,PM2.5导致的相对风险(RR)上升至1.0149(95%CI1.0054,1.0243),四川省O3导致的RR上升至1.0089(95%CI1.0030,1.0148)。我们发现四川省的心血管死亡率和空气污染呈显著正相关。使用高分辨率曝光将更准确地估计空气污染对CVD的影响。TYAP之后,SCB中老年人因PM2.5导致的心血管死亡风险估计降低,四川省O3引起的风险增加。
    Air pollution is a major risk factor of cardiovascular disease (CVD). To date, limited studies have estimated the effects of ambient air pollution on CVD mortality using high-resolution exposure assessment, which might fail to capture the spatial variation in exposure and introduce bias in results. Besides, the three-year action plan (TYAP, 2018-2020) was released; thus, the constitution and health effect of air pollutants may have changed. In this study, we estimated the short-term effect exposed to particulate matters with parameter less than 2.5 µm (PM2.5) and ozone (O3) with 0.05° × 0.05° resolution on CVD mortality and measured the influence of TYAP in the associations. We used random forest models with spatial weight matrices to attain high-resolution pollutant concentrations and conditional Poisson regression to assess the relationship between air pollution and cardiovascular mortality. With an increase of 10 µg/m3 in PM2.5 and O3 during 2018-2021 in the Sichuan Basin (SCB), CVD mortality increased 1.0134 (95% CI 1.0102, 1.0166) and 1.0083 (95% CI 1.0060, 1.0107), respectively, using high-resolution air pollutant concentration, comparing to 1.0070 (95% CI 1.0052, 1.0087) and 1.0057 (95% CI 1.0037, 1.0078) using data from air quality monitoring stations (AQMs). After TYAP, the relative risk (RR) due to PM2.5 rose up to 1.0149 (95% CI 1.0054, 1.0243), and the RR due to O3 rose up to 1.0089 (95% CI 1.0030, 1.0148) in Sichuan Province. We found significantly positive association of cardiovascular mortality and air pollution in Sichuan Province. And using high-resolution exposure would be more accurate to estimate the effect of air pollution on CVD. After TYAP, the cardiovascular mortality risk estimation due to PM2.5 decreased in elderly in SCB, and the risk due to O3 increased in Sichuan Province.
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  • 文章类型: Journal Article
    为农业系统设定氮(N)排放目标对于防止空气和地下水污染至关重要。然而,这种目标很少在县一级定义。在这项研究中,我们采用预测和回归方法建立了基于人类健康的曲周县空气和地下水水质氮指标,位于华北平原。通过采用世界卫生组织(WHO)的PM2.5浓度的I期标准(35μgm-3)和饮用水中硝酸盐的11.3mgNO3--NL-1的标准,我们发现,到2050年,整个县的氨气(NH3)排放量必须减少至少3.2千吨,才能达到世界卫生组织的PM2.5第一阶段标准。此外,控制其他污染物如二氧化硫(SO2)和氮氧化物(NOx)是必要的,2017-2050年期间,所需的减排量从16%到64%不等。此外,达到地下水质量标准,到2050年,硝酸盐氮(NO3--N)淋溶到地下水中的时间不应超过0.8千吨。要实现这一目标,2050年衢州农业NH3排放量和NO3-N淋洗量要比2017年各自水平减少50%(5.0和2.1千吨,分别)。我们开发的方法和由此产生的N排放目标可以通过促进控制策略的设计以最大程度地减少农业N损失来支持环境友好型农业的发展。
    Setting nitrogen (N) emission targets for agricultural systems is crucial to prevent to air and groundwater pollution, yet such targets are rarely defined at the county level. In this study, we employed a forecasting-and-back casting approach to establish human health-based nitrogen targets for air and groundwater quality in Quzhou county, located in the North China Plain. By adopting the World Health Organization (WHO) phase I standard for PM2.5 concentration (35 μg m-3) and a standard of 11.3 mg NO3--N L-1 for nitrate in drinking water, we found that ammonia (NH3) emissions from the entire county must be reduced by at least 3.2 kilotons year-1 in 2050 to meet the WHO\'s PM2.5 phase I standard. Additionally, controlling other pollutants such as sulfur dioxide (SO2) and nitrogen oxides (NOx) is necessary, with required reductions ranging from 16% to 64% during 2017-2050. Furthermore, to meet the groundwater quality standard, nitrate nitrogen (NO3--N) leaching to groundwater should not exceed 0.8 kilotons year-1 by 2050. Achieving this target would require a 50% reduction in NH3 emissions and a 21% reduction in NO3--N leaching from agriculture in Quzhou in 2050 compared to their respective levels in 2017 (5.0 and 2.1 kilotons, respectively). Our developed method and the resulting N emission targets can support the development of environmentally-friendly agriculture by facilitating the design of control strategies to minimize agricultural N losses.
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  • 文章类型: Journal Article
    在1990年代,在伊比利亚半岛东部建立了使用附生地衣多样性对森林进行生物监测的采样网络。该地区记录了安道尔热电厂风的空气污染影响,以及来自当地和长途运输的光氧化剂和氮沉积。1997年,通过计算大气纯度指数对地衣群落的状况进行了评估。此外,根据症状演变的速度及其在该地区的广泛分布,在预先选择的9种大型地衣中记录了可见的形态损伤症状。火电厂自2020年以来一直关闭和不活跃。在2022年,将近25年后,重新评估了以前建立的生物监测的七个站。为了比较1997年和2022年获得的结果,使用了相同的方法,和空气质量站的数据也包括在内。我们测试了,通过将创新方法(NIRS)集成到生物监测工具中,可以做出综合反应。结果表明,在1997年研究的目标地衣物种中,生物多样性普遍减少,而损害症状普遍增加,这似乎是多因素反应的结果。
    In the 1990s, a sampling network for the biomonitoring of forests using epiphytic lichen diversity was established in the eastern Iberian Peninsula. This area registered air pollution impacts by winds from the Andorra thermal power plant, as well as from photo-oxidants and nitrogen depositions from local and long-distance transport. In 1997, an assessment of the state of lichen communities was carried out by calculating the Index of Atmospheric Purity. In addition, visible symptoms of morphological injury were recorded in nine macrolichens pre-selected by the speed of symptom evolution and their wide distribution in the territory. The thermal power plant has been closed and inactive since 2020. During 2022, almost 25 years later, seven stations of this previously established biomonitoring were revaluated. To compare the results obtained in 1997 and 2022, the same methodology was used, and data from air quality stations were included. We tested if, by integrating innovative methodologies (NIRS) into biomonitoring tools, it is possible to render an integrated response. The results displayed a general decrease in biodiversity in several of the sampling plots and a generalised increase in damage symptoms in the target lichen species studied in 1997, which seem to be the consequence of a multifactorial response.
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