由于编码刺突糖蛋白的基因中的突变,SARS-CoV-2的关注的遗传变体(VoC)已经在世界范围内出现。我们使用Nextstrain服务器上可用的数据,对SARS-CoV-2的显着变异进化枝中的刺突蛋白突变进行了全面分析。我们选择了各种突变,即,A222V,N439K,N501Y,L452R,Y453F,E484K,K417N,T478K,L981F,L212I,N856K,T547K,G496S,和Y369C用于本研究。这些突变是根据它们的全局熵评分选择的,出现,传播,传输,以及它们在刺突受体结合域(RBD)中的位置。这些突变的相对丰度用全局突变D614G作图作为参考。我们的分析表明,与D614G一起,新的全球突变迅速出现,正如最近世界各地发生的COVID-19浪潮所报道的那样。这些突变可能是传播的必要手段,传染性,毒力,和宿主免疫系统对SARS-CoV-2的逃避。这些突变对疫苗有效性的可能影响,抗原多样性,抗体相互作用,蛋白质稳定性,RBD灵活性,并在计算机上研究了人细胞受体ACE2的可及性。总的来说,本研究可以帮助研究人员设计下一代疫苗和生物治疗药物来对抗COVID-19感染。
Genetic variant(s) of concern (
VoC) of SARS-CoV-2 have been emerging worldwide due to mutations in the gene encoding spike glycoprotein. We performed comprehensive analyses of spike protein mutations in the significant variant clade of SARS-CoV-2, using the data available on the Nextstrain server. We selected various mutations, namely, A222V, N439K, N501Y, L452R, Y453F, E484K, K417N, T478K, L981F, L212I, N856K, T547K, G496S, and Y369C for this study. These mutations were chosen based on their global entropic score, emergence, spread, transmission, and their location in the spike receptor binding domain (RBD). The relative abundance of these mutations was mapped with global mutation D614G as a reference. Our analyses suggest the rapid emergence of newer global mutations alongside D614G, as reported during the recent waves of COVID-19 in various parts of the world. These mutations could be instrumentally imperative for the transmission, infectivity, virulence, and host immune system\'s evasion of SARS-CoV-2. The probable impact of these mutations on vaccine effectiveness, antigenic diversity, antibody interactions, protein stability, RBD flexibility, and accessibility to human cell receptor ACE2 was studied in silico. Overall, the present study can help researchers to design the next generation of vaccines and biotherapeutics to combat COVID-19 infection.