UNASSIGNED: To investigate the effect of microencapsulated transgenic bone marrow mesenchymal stem cells (BMSCs) transplantation on early steroid induced osteonecrosis of femoral head (SONFH) in rabbits.
UNASSIGNED: Alginate poly- L-lysine-sodium alginate (APA) microencapsulated transgenic BMSCs with high expression of Foxc2 were prepared by high-voltage electrostatic method. Part of the cells were cultured in osteoblasts and observed by alizarin red staining at 2 and 3 weeks. Forty New Zealand white rabbits were used to prepare SONFH models by using hormone and endotoxin. Thirty two rabbits who were successful modeling were screened out by MRI and randomly divided into 4 groups (groups A, B, C and D, n=8); another 6 normal rabbits were taken as normal control (group E). The rabbits in group A did not receive any treatment; and in groups B, C, and D were injected with normal saline, allogeneic BMSCs, and APA microencapsulated transgenic BMSCs respectively after core decompression. At 6 and 12 weeks after operation, specimens of femoral head were taken for HE staining to observe bone ingrowth; the expressions of osteocalcin (OCN), peroxisome proliferative activated receptor γ 2 (PPARγ-2), and vascular endothelial growth factor (VEGF) proteins were observed by immunohistochemistry staining. At 12 weeks after operation, the bone microstructure was observed by transmission electron microscope, and the maximum compressive strength and average elastic modulus of cancellous bone and subchondral bone were measured by biomechanics.
UNASSIGNED: After 2 and 3 weeks of induction culture, alizarin red staining showed the formation of calcium nodules, and the number of calcium nodules increased at 3 weeks when compared with 2 weeks. The rabbits in each group survived until the experiment was completed. Compared with groups A, B, and C, the trabeculae of group D were more orderly, the empty bone lacunae were less, there were abundant functional organelles, and obvious osteogenesis was observed, and the necrotic area was completely repaired at 12 weeks. Immunohistochemical staining showed that, at 6 and 12 weeks after operation, the expressions of OCN and VEGF in groups A, B, and C were significantly lower than those in groups D and E, while those in groups B and C were significantly higher than those in group A, and in group E than in group D ( P<0.05). The expression of PPARγ-2 was significantly higher in groups A, B, and C than in groups D and E, and in group A than in groups B and C, and in group D than in group E ( P<0.05). At 12 weeks after operation, biomechanical test showed that the average elastic modulus and maximum compressive strength of cancellous bone and subchondral bone in groups D and E were significantly higher than those in groups A, B, and C ( P<0.05); there was no significant difference between groups A, B, and C and between groups D and E ( P>0.05).
UNASSIGNED: In vivo transplantation of microencapsulated transgenic BMSCs can repair early SONFH in rabbits.
UNASSIGNED: 探讨微囊化转基因 BMSCs 移植修复兔早期激素性股骨头坏死(steroid-induced osteonecrosis of femoral head,SONFH)的疗效。.
UNASSIGNED: 采用高压静电法制备海藻酸钠-多聚赖氨酸-海藻酸钠(sodium alginate-poly- L-lysine-sodium alginate,APA)微囊化高表达 Foxc2 转基因 BMSCs,取部分细胞成骨诱导培养,2、3 周时茜素红染色观察。取 40 只新西兰大白兔,采用激素加内毒素方法制备 SONFH 模型,经 MRI 筛选造模成功 32 只,将其随机分为 A、B、C、D 4 组( n=8);另取 6 只正常兔作为正常对照组(E 组)。A 组模型动物不作任何处理;B、C、D 组动物双侧后肢股骨头行髓芯减压后,分别于减压区注入生理盐水、同种异体 BMSCs、APA 微囊化转基因 BMSCs。术后 6、12 周取股骨头标本行 HE 染色,观察骨长入情况;免疫组织化学染色观察骨钙蛋白(osteocalcin,OCN)、过氧化酶体增殖剂激活受体 γ 2(peroxisome proliferative activated receptor γ 2,PPARγ-2)和 VEGF 蛋白表达;术后 12 周透射电镜观察骨微结构,生物力学测定松质骨及软骨下骨最大压缩强度及平均弹性模量。.
UNASSIGNED: 诱导培养 2、3 周,茜素红染色后均可见钙结节形成,且 3 周时数量较 2 周时增加。各组实验动物均存活至实验完成。组织学及透射电镜观察示,与 A、B、C 组比较,D 组骨小梁排列较整齐,骨空骨陷窝较少,有丰富的功能细胞器,可见明显成骨,且 12 周时坏死区被完全修复。免疫组织化学染色示,术后 6、12 周,A、B、C 组 OCN 及 VEGF 表达均低于 D、E 组,B、C 组高于 A 组,E 组高于 D 组,差异均有统计学意义( P<0.05)。A、B、C 组 PPARγ-2 表达高于 D、E 组,A 组高于 B、C 组,D 组高于E 组,差异均有统计学意义( P<0.05)。术后 12 周生物力学测试显示,D、E 组股骨头松质骨、软骨下骨平均弹性模量和最大压缩强度均高于 A、B、C 组( P<0.05);A、B、C 组间及 D、E 组间比较差异均无统计学意义( P>0.05)。.
UNASSIGNED: 微囊化转基因 BMSCs 体内移植可修复兔早期 SONFH。.