背景:脂肪组织(AT)的定性分析是几种疾病研究和临床应用的令人兴奋的领域,并且随着超重和肥胖者研究的定量方法而出现。虽然已经报道了类固醇代谢在多囊卵巢综合征(PCOS)女性中的重要性,关于AT在PCOS孕妇中的有效作用的数据有限。这项研究的目的是确定脂肪酸(FA)谱与PCOS腹部皮下AT中14种类固醇基因表达的相关性。非PCOS孕妇。
方法:在本病例对照研究中,收集了36例非PCOS孕妇和12例PCOS孕妇(3∶1比例控制:病例)剖宫产的AT样本.在R3.6.2软件上使用Pearson相关分析进行基因表达靶标与不同特征的关系。R工具中的ggplot2包用于绘制图。
结果:年龄(31.4岁和31.5岁,P=0.99),体重指数(BMI)(怀孕前26和26.5kg。m-2,P=0.62)和交货日(30.1和31,P=0.94),妊娠期(264天和267天,非PCOS和PCOS孕妇的P=0.70)和产次(1.4和1.4,P=0.42)相似。非PCOS孕妇中类固醇急性调节因子(STAR)和11β-羟基类固醇脱氢酶(11BHSD2)的表达与二十碳五烯酸(EPA,C20:5n-3,r=0.59,P=0.001)和(r=0.66,P=0.001),分别。在所有参与者中,STARmRNA水平与EPA脂肪酸浓度的相关性最大(P=0.001,r=0.51)。
结论:我们的研究结果表明,孕妇体内类固醇代谢与脂肪酸之间存在联系,特别是对于omega-3FA和参与皮下AT类固醇生成第一步的基因。这些发现值得进一步研究。
BACKGROUND: The qualitative analysis of adipose tissue (AT) is an exciting area for research and clinical applications in several diseases and it is emerging along with the quantitative approach to research on overweight and obese people. While the importance of steroid metabolism in women with polycystic ovary syndrome (PCOS) has been reported, limited data exists on the effective roles of AT in pregnant women suffering from PCOS. The aim of this study was to determine association of fatty acid (FA) profiles with expression of 14 steroid genes in abdominal subcutaneous AT of PCOS vs. non-PCOS pregnant women.
METHODS: In this
case-control study, the AT samples of 36 non-PCOS pregnant women and 12 pregnant women with PCOS (3:1 ratio control:
case) who underwent cesarean section were collected. Relationship of expressing gene targets and different features were performed using Pearson correlation analysis on the R 3.6.2 software. The ggplot2 package in R tool was used to draw the plots.
RESULTS: Age (31.4 and 31.5 years, P=0.99), body mass index (BMI) (prior pregnancy 26 and 26.5 kg.m-2, P=0.62) and at delivery day (30.1 and 31, P=0.94), gestational period (264 and 267 days, P=0.70) and parity (1.4 and 1.4, P=0.42) of non-PCOS and PCOS pregnant women were similar. Expression of steroidogenic acute regulator (STAR) and 11β-Hydroxysteroid dehydrogenase (11BHSD2) in non-PCOS pregnant women showed the highest association with eicosapentaenoic acid (EPA, C20:5 n-3, r=0.59, P=0.001) and (r=0.66, P=0.001), respectively. In the all participants, STAR mRNA level showed the greatest association with the EPA fatty acid concentration (P=0.001, r=0.51).
CONCLUSIONS: Our results showed a link between the genes involved in steroid metabolism and fatty acids in AT of pregnant women, especially for omega-3 FA and the gene involved in the first step of
steroidogenesis in subcutaneous AT. These findings warrant further studies.