■人参已被用作治疗许多疾病和维持一般健康的传统药物。以前,我们表明人参在去卵巢小鼠模型中没有表现出雌激素特性。然而,类固醇生成的破坏仍然可能导致间接激素活性。
■根据OECD检测内分泌干扰化学物质的指南:测试指南(TG)No.456(用于检测类固醇生成特性的体外测定方法)和TGNo.440(具有子宫营养性质的化学品的体内短期筛选方法)。
■韩国红参(KRG)和人参皂苷Rb1、Rg1和Rg3不干扰根据TG456在H295细胞中检测的雌激素和睾酮激素合成。对卵巢切除小鼠的KRG处理未显示子宫重量的显著变化。此外,血清雌激素和睾酮水平未因摄入KRG而改变。
■这些结果清楚地表明,没有与KRG相关的类固醇生成活性,也没有KRG破坏下丘脑-垂体-性腺轴。将进行额外的测试以追求人参的细胞分子靶标以显示作用模式。
UNASSIGNED: Ginseng has been used as a traditional medicine for treatment of many diseases and for general health maintenance. Previously, we showed that ginseng did not demonstrate estrogenic property in ovariectomized mouse model. However, it is still possible that disruption of
steroidogenesis leading to indirect hormonal activity.
UNASSIGNED: The hormonal activities were examined in compliance with OECD
guidelines for detecting endocrine disrupting chemicals: test
guideline (TG) No. 456 (an in vitro assay method for detecting
steroidogenesis property) and TG No. 440 (an in vivo short-term screening method for chemicals with uterotrophic property).
UNASSIGNED: Korean Red Ginseng (KRG) and ginsenosides Rb1, Rg1, and Rg3 did not interfere with estrogen and testosterone hormone synthesis as examined in H295 cells according to TG 456. KRG treatment to ovariectomized mice did not show a significant change in uterine weight. In addition, serum estrogen and testosterone levels were not change by KRG intake.
UNASSIGNED: These results clearly demonstrate that there is no steroidogenic activity associated with KRG and no disruption of the hypothalamic-pituitary-gonadal axis by KRG. Additional tests will be performed in pursuit of cellular molecular targets of ginseng to manifest mode of action.