植酸(PA)是一种强抗营养因子,在对抗活性氧中具有关键的抗氧化作用。尽管低植酸(lpa)突变体的潜在好处,PA的减少引起多效性效应,例如,与种子老化相关的种子发芽和生存力损失减少。当前的研究评估了一系列历史上自然老化的种子,并表明与野生型相比,lpa1-1种子的老化速度更快。为了模仿自然衰老,本研究建立了不同温度下的加速老化处理。发现将种子在57°C下孵育24小时,野生型萌发率为82.4%,lpa1-1萌发率为40%。当前的研究还假设了两种可能的解决方案来克服这些问题:(1)经典育种用于构成携带lpa1-1突变的合成种群,基因推动花青素在胚胎中的积累(R-navajo等位基因)。结果表明,在lpa1-1基因型中存在R-navajo不能提高发芽性(-20%),但这种方法可能有助于提高非突变基因型的发芽性(+17%)。(2)此外,在lpa1-1和野生型种子上测试了水引发作用,lpa1-1的发芽率提高了20%,表明种子引发在恢复发芽方面具有积极作用。此外,数据强调了在水引发治疗之前和之后代谢组的代谢差异,表明发芽的差异也可能是由突变引起的代谢组成差异介导的。
Phytic acid (PA) is a strong anti-nutritional factor with a key antioxidant role in countering reactive oxygen species. Despite the potential benefits of low phytic acid (lpa) mutants, the reduction of PA causes pleiotropic effects, e.g., reduced seed germination and viability loss related to seed ageing. The current
study evaluated a historical series of naturally aged seeds and showed that lpa1-1 seeds aged faster as compared to wildtype. To mimic natural ageing, the present
study set up accelerated ageing treatments at different temperatures. It was found that incubating the seeds at 57 °C for 24 h, the wildtype germinated at 82.4% and lpa1-1 at 40%. The current
study also hypothesized two possible solutions to overcome these problems: (1) Classical breeding was used to constitute synthetic populations carrying the lpa1-1 mutation, with genes pushing anthocyanin accumulation in the embryo (R-navajo allele). The outcome showed that the presence of R-navajo in the lpa1-1 genotype was not able to improve the germinability (-20%), but this approach could be useful to improve the germinability in non-mutant genotypes (+17%). (2) In addition, hydropriming was tested on lpa1-1 and wildtype seeds, and germination was improved by 20% in lpa1-1, suggesting a positive role of seed priming in restoring germination. Moreover, the data highlighted metabolic differences in the metabolome before and after hydropriming treatment, suggesting that the differences in germination could also be mediated by differences in the metabolic composition induced by the mutation.