体内和微观化学分析方法有可能提高我们对植物代谢和发育的认识。台式微探针X射线荧光光谱法(μ-XRF)为应对这一挑战提供了巨大的潜力。采用直径为30μm和1mm的激发束来解决种子技术中的问题,植物病理学,植物生理学,和生物修复。在几种具有农艺意义的情况下分析了不同的元素:(i)μ-XRF产生定量图的例子,揭示了普通豆(菜豆)种子中锌的空间分布。(ii)每天在受炭疽病感染的大豆叶(Glycinemax)上记录的化学图像显示磷,硫磺,和钙倾向于集中在病斑。(iii)在普通P的茎的体内测量表明,在根暴露下,锰的吸收和运输速度比铁快近10倍。(四)定量图显示,桉树杂种叶片中的铅分布不均匀,这种元素主要积累在叶缘和中脉,铅热点达到13,400mg铅kg-1新鲜组织重量。这些案例研究强调了μ-XRF对新鲜和活植物组织进行定性和定量元素分析的能力。因此,它可以无损地实时探测动态生物现象。
In vivo and micro chemical analytical methods have the potential to improve our understanding of plant metabolism and development. Benchtop microprobe X-ray fluorescence spectroscopy (μ-XRF) presents a huge potential for facing this challenge. Excitation beams of 30 μm and 1 mm in diameter were employed to address questions in seed technology, phytopathology, plant physiology, and bioremediation. Different elements were analyzed in several situations of agronomic interest: (i) Examples of μ-XRF yielding quantitative maps that reveal the spatial distribution of zinc in common beans (Phaseolus vulgaris) primed seeds. (ii) Chemical images daily recorded at a soybean leaf (Glycine max) infected by anthracnose showed that phosphorus, sulfur, and calcium trended to concentrate in the disease spot. (iii) In vivo measurements at the stem of P. vulgaris showed that under root exposure, manganese is absorbed and transported nearly 10-fold faster than iron. (iv) Quantitative maps showed that the lead distribution in a leaf of Eucalyptus hybrid was not homogenous, this element accumulated mainly in the leaf border and midrib, the lead hotspots reached up to 13,400 mg lead kg-1 fresh tissue weight. These
case studies highlight the ability of μ-XRF in performing qualitative and quantitative elemental analysis of fresh and living plant tissues. Thus, it can probe dynamic biological phenomena non-destructively and in real time.