利用再生水进行城市景观的生态补给对缓解城市水资源短缺起着至关重要的作用。在银川,我们研究了用黄河或再生水补给城市河流对微生物群落的丰度和多样性的影响。本研究旨在支持再生水的有效利用。我们监测了六个地点:三个在再生水补给区(Lucaowa入口(ZLJ),Lucaowa频道(ZLH),和卢考瓦出口(ZLC))和三个黄河水补给区(宁城船闸(FNCZ),青凤街(FQFJ),和莱佛斯(FLFS))。各种指标(pH,浊度,温度(T),溶解氧(DO),电导率(EC),化学需氧量(COD),总磷(TP),总氮(TN),氨氮(NH3-N),和硝酸盐氮(NO3-N))用于评估水质。使用16SrRNA高通量测序评估了微生物群落的丰度和多样性。结果表明,在整个监测期间,与黄河水补给区相比,再生水补给区表现出更高的水透明度和更大的微生物群落丰度和多样性。然而,再生水补给区也显示出明显较高的氮水平,磷,有机物,和导电性,随着Firmicutes的增加。季节性变化显著影响水质因子,显著影响蓝细菌和弯曲杆菌种群,正如RDA分析所证明的那样,微生物群落与环境因子密切相关。进一步的比较分析表明,再生水补给区的红细胞细菌占优势。而放线菌,浮游生物,和曲霉属。在黄河流域补水区更为显著。微生物功能的预测分析表明,再生水补给区的碳氮循环相关功能更加丰富,说明再生水补给可以提高水体的自净能力。
The ecological recharge of urban landscapes with reclaimed water plays a crucial role in alleviating urban water shortage. In Yinchuan, we examined the effects of recharging urban rivers with either Yellow River or reclaimed water on the abundance and diversity of microbial communities. This study aimed to support the effective utilization of reclaimed water. We monitored six sites: three in the reclaimed water recharge area (Lucaowa inlet (ZLJ), Lucaowa channel (ZLH), and Lucaowa outlet (ZLC)) and three in the Yellow River water recharge area (Ningcheng lock (FNCZ), Qingfengjie (FQFJ), and Laifosi (FLFS)). Various indicators (pH, turbidity, temperature (T), dissolved oxygen (DO), electrical conductivity (EC), chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN), ammonia nitrogen (NH3-N), and nitrate nitrogen (NO3-N)) were used to assess the water quality. The microbial community abundance and diversity were evaluated using 16S rRNA high-throughput sequencing. The results indicated that throughout the monitoring period, the reclaimed water recharge area exhibited increased water transparency and greater microbial community abundance and diversity than the Yellow River water recharge area. However, the reclaimed water recharge area also showed significantly higher levels of nitrogen, phosphorus, organic matter, and electrical conductivity, along with an increase in Firmicutes. Seasonal changes significantly influenced water quality factors, significantly affecting Cyanobacteria and Campylobacter populations, as demonstrated by RDA analysis, which showed a close relationship between microbial communities and environmental factors. Further comparative analysis revealed that erythrocytic bacteria were predominant in the reclaimed water recharge area, whereas Actinobacteria, Planktonia, and Aspergillus spp. were more significant in the Yellow River water recharge area. Predictive analysis of microbial functions suggested that carbon and nitrogen cycle-related functions were more abundant in the reclaimed water recharge area, indicating that reclaimed water recharge could improve the self-purification capacity of the water body.