本研究旨在研究波斯松鼠(Sciurusanomalus)的小脑组织学,采用免疫组织化学染色和透射电镜(TEM)。使用了4只成年波斯松鼠(2只雄性和2只雌性)的大脑。对于一般组织学,应用结晶紫和Luxol快速蓝色染色剂。对于免疫组织化学检查,髓鞘相关糖蛋白(MAG),神经丝200(NF200),CalbindinD28K,和胶质纤维相关蛋白(GFAP)进行了研究。小脑皮层由三个传统细胞层组成,包括分子,颗粒和浦肯野细胞(Pc)层。在Pc的细胞质中检测到许多Nissl体,赋予细胞质紫色或蓝色外观。在TEM的水平,浦肯野细胞通过其大的常染色质核与凹陷的质膜鉴定。细胞质高电子致密,含有发育良好的粗面内质网(Er),光滑的呃,核周区域的高尔基体,许多线粒体呈椭圆形和球形,游离核糖体,溶酶体分散在整个细胞质中。钙结合蛋白D的表达率28K,MAG,GFAP,NF200被确定为49%,24%,71%,分别为43%。波斯松鼠的小脑具有与其他哺乳动物相似的共同结构。然而,在TEM的水平,观察到一些独特的结构特征。这些结果可能被视为一组基本信息,用于更详细的未来研究,以调查小脑的组织生理学及其在波斯松鼠中的病理变化,以及在啮齿动物中的比较目的。
The present
study was aimed to investigate the cerebellum histology in the Persian squirrel (Sciurus anomalus), using immunohistochemical staining and transmission electron microscopy (TEM). The brain of 4 adult Persian squirrels (2 males and 2 females) were used. For general histology, crystal violet and Luxol fast blue stains were applied. For immunohistochemical examination, myelin associated glycoprotein (MAG), neurofilament 200 (NF200), Calbindin D 28 K, and glial fibrillary associated protein (GFAP) were investigated. The cerebellar cortex was composed of three traditional cellular layers, including molecular, granular and Purkinje cell (Pc) layers. Numerous Nissl bodies detected in the cytoplasm of Pc, which given a purple or blue appearance to the cytoplasm. At the level of TEM, Purkinje cells were identified by their large euchromatin nucleus with an indented plasma membrane. The cytoplasm was high electron dense containing well-developed rough endoplasmic reticulum (Er), smooth Er, Golgi apparatus in perinuclear region, numerous mitochondria in oval and spherical shapes, free ribosomes, and lysosomes were dispersed throughout the cytoplasm. The expression rate of calbindin D 28 K, MAG, GFAP, and NF200 was determined 49%, 24%, 71%, and 43% respectively. The cerebellum of the Persian squirrel has a common structure similar to those reported in other mammals. However, at the level of TEM, some unique structural features were observed. These results might be taken as a set of basic information for more detailed future studies investigating histophysiology of the cerebellum and its pathological changes in the Persian squirrel as well as for comparative purposes in rodent species.