蛋白质聚集在各个领域都存在问题,在常规实验期间经常发生聚集。这项研究表明,四甘醇(TEG)和四甘醇二甲醚(TEGDE)作为聚集抑制剂,具有不同于典型添加剂的独特性质,以防止蛋白质聚集,例如精氨酸(Arg)和NaCl。加入TEG和TEGDE可以很好地抑制α-胰凝乳蛋白酶的热聚集。有趣的是,当温度从65°C移至85°C时,Arg和NaCl对热聚集的抑制作用几乎没有变化。随着温度的升高,TEG和TEGDE均显着降低了聚集速率。请注意,TEG和TEGDE的效果高于75°C以上的Arg。TEG和TEGDE的这种温度依赖性行为为开发在高温下使用的聚集抑制剂提供了一种新颖的设计指南。即,环氧乙烷基团的重要性。
Protein aggregation is problematic in various fields, where aggregation can frequently occur during routine experiments. This study showed that tetraethylene glycol (TEG) and tetraethylene glycol dimethyl ether (TEGDE) act as aggregation suppressors that have different unique properties from typical additives to prevent protein aggregation, such as arginine (Arg) and NaCl. Thermal aggregation of α-chymotrypsin was well suppressed with the addition of both TEG and TEGDE. Interestingly, the suppressive effects of Arg and NaCl on thermal aggregation were almost unchanged when temperature was shifted from 65°C to 85°C, whereas both TEG and TEGDE significantly decreased the aggregation rate with increasing temperature. Note that the effects of TEG and TEGDE were higher than Arg above 75°C. This temperature-dependent behavior of TEG and TEGDE provides a novel design
guideline to develop aggregation suppressors for use at high temperature, i.e., the importance of the ethylene oxide group.