维生素D的活性形式具有免疫调节和抗炎作用。维生素D与类风湿性关节炎(RA)的发病机理有关,其缺乏会导致炎症增加。此外,它的生产取决于钙的浓度,磷,和甲状旁腺激素(PTH)。细胞因子介导RA滑膜中的炎症。这项研究评估了维生素D,RA患者的介质和促炎细胞因子。
在一项病例对照研究中,招募了来自KomfoAnokye教学医院风湿病诊所的78名RA患者和60名健康献血者。化学分析仪和酶联免疫吸附测定试剂盒用于测量生化参数和细胞因子。
我们发现白细胞介素(IL)-1β的水平明显较高,干扰素γ(IFN-γ),与对照组相比,RA患者的肿瘤坏死因子-α(TNF-α)和肿瘤坏死因子-α(p<0.05)。完整甲状旁腺激素(iPTH)与IL-10呈显著正相关(r=.30,p<.05),IL-6呈负相关(r=-0.28,p>.05)。IL-1β(r=-0.25,p>0.05),TNF-α(r=-0.26,p>0.05),IFN-γ(r=-0.24,p>0.05),和iPTH。IL-1β之间呈显著负相关(r=-0.33,p<0.05),IFN-γ(r=-0.29,p<0.05),和钙。
PTH降低,钙,和磷与更高水平的促炎细胞因子有关,这可能会使RA疾病恶化。因此,维生素D不是RA中促炎细胞因子的独立调节剂。
The active form of vitamin D has immunomodulatory and anti-inflammatory effect. Vitamin D is implicated in pathogenesis of rheumatoid arthritis (RA) and its deficiency leads to increased inflammation. Moreover, its production is dependent on concentration of calcium, phosphorus, and parathyroid hormone (PTH). Cytokines mediates inflammation in RA synovium. This study evaluated vitamin D, its mediators and proinflammatory cytokines among RA patients.
In a
case-control study, 78 RA patients from Komfo Anokye Teaching Hospital rheumatology clinic and 60 healthy blood donors were recruited. Chemistry analyzer and enzyme-linked immunosorbent assay kits were used to measure biochemical parameters and cytokines.
We found significantly higher levels of interleukin (IL)-1β, interferon gamma (IFN-γ), and tumor necrosis factor-α (TNF-α) in RA patients compared with controls (p < .05). There was a significant positive correlation between intact parathyroid hormone (iPTH) and IL-10 (r = .30, p < .05) and a negative correlation between IL-6 (r = -0.28, p > .05), IL-1β (r = -0.25, p > .05), TNF-α (r = -0.26, p > .05), IFN-γ (r = -0.24, p > .05), and iPTH. There was a significant negative correlation between IL-1β (r = -0.33, p < .05), IFN- γ (r = -0.29, p < .05), and calcium.
Reduced PTH, calcium, and phosphorus is associated with higher levels of proinflammatory cytokines which may worsen RA disease condition. Vitamin D is therefore not an independent regulator of proinflammatory cytokines in RA.