血清学交叉反应性,正痘病毒(OPXV)感染的标志,使感染的物种特异性诊断困难。在这项研究中,我们使用天花病毒蛋白质组芯片来表征和区分针对非牛痘OPXV感染和天花疫苗接种的抗体应答.2例新发现的OPXV感染患者的概况,Akhmeta病毒,表现出更高强度和更广泛的病毒蛋白识别的抗体应答,并且包括用作疫苗的牛痘病毒株未编码的B21/22家族糖蛋白。另一例Akhmeta病毒,或非牛痘OPXV感染,通过对无疫苗接种史或不确定疫苗接种史以及近期无感染史的个体进行社区监测来确定。结果证明微阵列用于抗体应答的高分辨率作图以确定OPXV暴露的性质的实用性。
Serologic cross-reactivity, a hallmark of orthopoxvirus (OPXV) infection, makes species-specific diagnosis of infection difficult. In this study, we used a variola virus proteome microarray to characterize and differentiate antibody responses to nonvaccinia OPXV infections from smallpox vaccination. The profile of 2
case patients infected with newly discovered OPXV, Akhmeta virus, exhibited antibody responses of greater intensity and broader recognition of viral proteins and includes the B21/22 family glycoproteins not encoded by vaccinia virus strains used as vaccines. An additional
case of Akhmeta virus, or nonvaccinia OPXV infection, was identified through community surveillance of individuals with no or uncertain history of vaccination and no recent infection. The results demonstrate the utility of microarrays for high-resolution mapping of antibody response to determine the nature of OPXV exposure.