Abstract：
An outbreak of a disease in camels with skin lesions was reported in Israel during 2016. To identify the etiological agent of this illness, we employed a multidisciplinary diagnostic approach. Transmission electron microscopy (TEM) analysis of lesion material revealed the presence of an orthopox-like virus, based on its characteristic brick shape. The virus from the skin lesions successfully infected chorioallantoic membranes and induced cytopathic effect in Vero cells, which were subsequently positively stained by an orthopox-specific antibody. The definite identification of the virus was accomplished by two independent qPCR, one of which was developed in this study, followed by sequencing of several regions of the viral genome. The qPCR and sequencing results confirmed the presence of camelpox virus (CMLV), and indicated that it is different from the previously annotated CMLV sequence available from GenBank. This is the first reported case of CMLV in Israel, and the first description of the isolated CMLV subtype.
摘要：
2016年，以色列报告了骆驼皮肤病变的疾病爆发。为了确定这种疾病的病因，我们采用了多学科诊断方法。病变物质的透射电子显微镜（TEM）分析显示存在类正痘病毒，根据其特征砖的形状。来自皮肤损伤的病毒成功感染绒毛尿囊膜，并在Vero细胞中诱导细胞病变效应，随后被正痘特异性抗体阳性染色。通过两个独立的qPCR对病毒进行了明确的鉴定，其中之一是在这项研究中开发的，然后对病毒基因组的几个区域进行测序。qPCR和测序结果证实了骆驼痘病毒（CMLV）的存在，并表明它不同于先前注释的可从GenBank获得的CMLV序列。这是以色列首例CMLV病例,和隔离的CMLV亚型的第一个描述。
