枇杷叶是枇杷果实生产的副产品。多糖是枇杷叶中的主要活性成分之一。在这项研究中,采用超声波辅助低共熔溶剂(DESs)提取法提取枇杷叶多糖。Further,采用S-8树脂和DEAE-52纤维素柱色谱对提取的枇杷叶粗多糖(CLLP)进行纯化分离,分别。此外,多糖对保存在17°C培养基中的公猪精液中精子活性的影响,进行了初步评价。DES,由氯化胆碱/乙二醇(1:6,摩尔比)组成,被证明是LLP提取的合适溶剂。优化的提取条件为含水量44%,液固比1:29(g/g),提取温度61°C和提取时间98分钟。在这些条件下,LLP产量为57.82±1.50mg/g。从CLLP中分离出均质多糖(LLP1-2,Mw:2.17×104Da)。它的总糖,糖醛酸和蛋白质含量为76.31±1.25%,14.19±0.67%和3.28±0.42%,分别。Further,800μg/mL的LLP1-2能有效增强精子的抗氧化活性。本研究为DESs和柱层析在多糖提取分离领域奠定了基础,证明LLP可以作为精子保存的天然抗氧化剂。
Loquat leaves are the by-product of loquat fruit production.
Polysaccharides are one of the main active ingredients in loquat leaves. In this
study,
polysaccharides were extracted from loquat leaves by ultrasonic-assisted deep eutectic solvents (DESs) extraction method. Further, the extracted crude loquat leaf
polysaccharides (CLLP) were purified and separated via S-8 resin and DEAE-52 cellulose column chromatography, respectively. Additionally, the effects of
polysaccharides on activity of sperm in boar semen preserved in medium at 17 °C, were evaluated preliminarily. DES, composed of choline chloride/ethylene glycol (1:6, molar ratio), was proved to be the suitable solvent for LLP extraction. The optimized extraction conditions were water content 44 %, liquid-solid ratio 1:29 (g/g), extraction temperature 61 °C and extraction time 98 min. Under these conditions, the LLP yield was 57.82 ± 1.50 mg/g. A homogeneous polysaccharide (LLP1-2, Mw: 2.17 × 104 Da) was isolated from CLLP. Its total sugar, uronic acid and protein contents were 76.31 ± 1.25 %, 14.19 ± 0.67 % and 3.28 ± 0.42 %, respectively. Further, 800 μg/mL LLP1-2 could effectively enhance the antioxidant activity of sperm. This
study laid a foundation for DESs and column chromatography in the field of polysaccharide extraction and separation, proving that LLP can be used as a natural antioxidant for sperm preservation.