■病态,代谢紊乱在多囊卵巢综合征(PCOS)中起着至关重要的作用.然而,没有确凿的证据表明脂质代谢物水平与PCOS风险有关.
■在这项研究中,我们使用122种脂质代谢物的全基因组关联研究(GWAS)遗传数据分配工具变量(IVs).PCOSGWAS来自对10,074例PCOS病例和103,164例对照的大规模荟萃分析。逆方差加权(IVW)分析是用于孟德尔随机化(MR)的主要方法。对于敏感性分析,CochranQ测试,MR-Egger截获,MR-PRESSO,遗漏分析,进行了Steiger测试。此外,我们进行了复制分析,荟萃分析,和代谢途径分析。最后,反向MR分析用于确定PCOS的发作是否影响脂质代谢产物.
■这项研究检测了与PCOS发病有遗传关联的血脂代谢产物和潜在代谢途径。IVW之后,敏感性分析,复制和荟萃分析,最终确定了PCOS的两种致病性脂质代谢产物:十六烷二酸(OR=1.85,95CI=1.27-2.70,P=0.001)和二高亚油酸(OR=2.45,95CI=1.30-4.59,P=0.005)。此外,研究发现PCOS可能通过不饱和脂肪酸生物合成和初级胆汁酸生物合成代谢途径介导。反向MR分析显示PCOS与2-十四烯酰基肉碱在遗传水平上存在因果关系(OR=1.025,95%CI=1.003-1.048,P=0.026)。
■遗传证据表明十六烷二酸和二高亚油酸与PCOS风险之间存在因果关系。这些化合物可能作为代谢生物标志物用于筛选PCOS和选择药物靶标。这些代谢途径的鉴定对探索PCOS的病理机制具有重要的指导意义。虽然进一步的研究是必要的确认。
UNASSIGNED: Pathologically, metabolic disorder plays a crucial role in polycystic ovarian syndrome (PCOS). However, there is no conclusive evidence lipid metabolite levels to PCOS risk.
UNASSIGNED: In this
study, genome-wide association
study (GWAS) genetic data for 122 lipid metabolites were used to assign instrumental variables (IVs). PCOS GWAS were derived from a large-scale meta-analysis of 10,074 PCOS cases and 103,164 controls. An inverse variance weighted (IVW) analysis was the primary methodology used for Mendelian randomization (MR). For sensitivity analyses, Cochran Q test, MR-Egger intercept, MR-PRESSO, leave-one-out analysis,and Steiger test were performed. Furthermore, we conducted replication analysis, meta-analysis, and metabolic pathway analysis. Lastly, reverse MR analysis was used to determine whether the onset of PCOS affected lipid metabolites.
UNASSIGNED: This
study detected the blood lipid metabolites and potential metabolic pathways that have a genetic association with PCOS onset. After IVW, sensitivity analyses, replication and meta-analysis, two pathogenic lipid metabolites of PCOS were finally identified: Hexadecanedioate (OR=1.85,95%CI=1.27-2.70, P=0.001) and Dihomo-linolenate (OR=2.45,95%CI=1.30-4.59, P=0.005). Besides, It was found that PCOS may be mediated by unsaturated fatty acid biosynthesis and primary bile acid biosynthesis metabolic pathways. Reverse MR analysis showed the causal association between PCOS and 2-tetradecenoyl carnitine at the genetic level (OR=1.025, 95% CI=1.003-1.048, P=0.026).
UNASSIGNED: Genetic evidence suggests a causal relationship between hexadecanedioate and dihomo-linolenate and the risk of PCOS. These compounds could potentially serve as metabolic biomarkers for screening PCOS and selecting drug targets. The identification of these metabolic pathways is valuable in guiding the exploration of the pathological mechanisms of PCOS, although further studies are necessary for confirmation.