Abstract：
To investigate the toxicological effects of polystyrene microplastics (PS-MPs), cadmium (Cd), and their combined contamination on the growth and physiological responses of V. faba seedlings, this experiment employed a hydroponic method. The Hoagland nutrient solution served as the control, changes in root growth, physiological and biochemical indicators of V. faba seedlings under different concentrations of PS-MPs (10, 100 mg/L) alone and combined with 0.5 mg/L Cd. The results demonstrated that the root biomass, root vitality, generation rate of superoxide radicals (O2·-), malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity increased with increasing concentration under the influence of PS-MPs alone, while the soluble sugar content and peroxidase (POD) activity decreased. In the combined treatment with Cd, the trends of these indicators are generally similar to the PS-MPs alone treatment group. However, root vitality and SOD activity showed an inverse relationship with the concentration of PS-MPs. Furthermore, laser confocal and electron microscopy scanning revealed that the green fluorescent polystyrene microspheres entered the root tips of the V. faba and underwent agglomeration in the treatment group with a low concentration of PS-MPs alone and a high concentration of composite PS-MPs with Cd.
摘要：
研究聚苯乙烯微塑料(PS-MPs)的毒理学效应,镉(Cd),以及它们对蚕豆幼苗生长和生理反应的综合污染，本实验采用水培方法。霍格兰营养液作为对照,根系生长的变化，不同浓度的PS-MPs(10,100mg/L)和与0.5mg/LCd复合处理对蚕豆幼苗生理生化指标的影响。结果表明,根系生物量,根活力，超氧自由基(O2·-)的产生速率，丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性随浓度的增加而增加,而可溶性糖含量和过氧化物酶（POD）活性下降。在与Cd的联合处理中，这些指标的趋势通常与单独PS-MPs治疗组相似.然而,根系活力和SOD活性与PS-MPs浓度呈负相关。此外，激光共聚焦和电子显微镜扫描显示，绿色荧光聚苯乙烯微球进入V.faba的根尖，并在低浓度的PS-MPs和高浓度的复合PS-MPs与Cd的处理组中发生团聚。
