目标:主要的环境问题,如青霉菌,在经济上在自然生态系统中发挥着至关重要的作用,生态,和医学上重要的微生物。在布什尔市尚未描述该属的生物多样性,伊朗。本研究基于使用部分b-微管蛋白序列的依赖培养方法和不依赖培养技术的青霉物种的空气生物多样性。
方法:通过使用具有高容量空气采样器的主动采样,总共选择了157株青霉菌分离株并筛选了表型特征。为了研究的目的,选择并通过分子分析鉴定了代表11种形态物种的46个菌株。
结果:根据调查结果,P.crustosum(18个分离株,39.1%)和产黄假单胞菌(15个分离株,32.6%)是最常见的分离物种,其次是假单胞菌,P.rubens,P.citrinum,意大利P.italicum(每2个分离株,4.3%),P.Olsonii,P.expansum,P.灰黄,P.palitans,和Polonicum(每1个分离株,2.1%)。除具有絮状菌落结构的产黄假单胞菌和扩展假单胞菌外,其余的孤立物种具有柔软的质地。
结论:这是伊朗南部首次发现从空气样本中分离出大量青霉菌株的报告,显示了最常见的分离物种。
OBJECTIVE: The main environmental saprobes, such as
Penicillium, play an essential role in natural ecosystems as economically, ecologically, and medically important microorganisms. Biodiversity of this genus has not been described in Bushehr city, Iran. The present
study is based on air biodiversity of
Penicillium species on culture-dependent approach and culture-independent technique using partial b-tubulin sequences.
METHODS: By using active sampling with a high volume air sampler, a total of 157
Penicillium isolates were selected and screened for phenotypic characters. For the purposes of the
study, 46 strains representative of 11 morphological species were selected and identified by molecular analysis.
RESULTS: Based on the findings, P. crustosum (18 isolates, 39.1%) and P. chrysogenum (15 isolates, 32.6%) were the most common isolated species, followed by P. brevicompactum, P. rubens, P. citrinum, P. italicum (each 2 isolates, 4.3%), P. olsonii, P. expansum, P. griseofulvum, P. palitans, and P. polonicum (each 1 isolate, 2.1%). Except for P. chrysogenum and P. expansum with floccose colony texture, the rest of the isolated species had velutinous texture.
CONCLUSIONS: This is the first report in southern Iran to identify a large number of
Penicillium strains isolated from air samples, showing P. crustosum and P. chrysogenum as the most common isolated species.