Abstract：
The galactomannan-based gel from Cassia grandis seeds was used to incorporate Penicillium sp. UCP 1286 and commercial collagenases. Experiments were carried out according to a 23-full factorial design to identify the most significant parameters for the incorporation process. The pH of the incorporation solution (pHi), stirring time (t), and initial protein concentration in the crude extract (PCi) were selected as the three independent variables, and the efficiency of collagenase incorporation (E) and collagenolytic activity (CA) after 360 min as the responses. pHi and PCi showed positive statistically significant effects on E, while CA was positively influenced by pHi and t, but negatively by PCi. The fungi collagenase was released from the gel following a pseudo-Fickian behavior. Additionally, no <76 % of collagenase was efficiently incorporated into the gel retaining a high CA (32.5-69.8 U/mL). The obtained results for the commercial collagenase (E = 93.88 %, CA = 65.8 U/mL, and n = 0.10) demonstrated a pseudo-Fickian behavior similar to the fungi-collagenase. The results confirm the biotechnological potential of the gel as an efficient matrix for the incorporation of catalytic compounds; additionally, the incorporation of collagenases was achieved by retaining the proteases CA and releasing them in a controlled manner.
摘要：
来自决明子种子的半乳甘露聚糖基凝胶用于掺入青霉属。UCP1286和商业胶原酶。根据23全因子设计进行实验,以确定掺入过程中最重要的参数。掺入溶液的pH值(pHi)，搅拌时间(t),和粗提物中的初始蛋白质浓度（PCi）被选择为三个独立变量，以及360分钟后胶原酶掺入效率（E）和胶原分解活性（CA）作为响应。pHi和PCi对E，虽然CA受到pHi和t的积极影响，但对PCi不利。真菌胶原酶在假Fickian行为后从凝胶中释放。此外,没有<76%的胶原酶有效掺入凝胶中,保持高CA(32.5-69.8U/mL)。商业胶原酶获得的结果(E=93.88%，CA=65.8U/mL,n=0.10）表现出与真菌-胶原酶相似的假Fickian行为。结果证实了凝胶作为掺入催化化合物的有效基质的生物技术潜力；此外，胶原酶的掺入是通过保留蛋白酶CA并以受控方式释放它们来实现的。
