背景:组织性肺炎(OP)是间质性肺炎类别中最常见和致命的疾病之一,还有肺癌.脂质代谢的重编程是包括癌症在内的许多疾病的新标志。心血管疾病,以及肝纤维化和硬化。由鞘氨醇和脂肪酸组成的神经酰胺水平增加,与急性和慢性肺部疾病的发展有关。然而,其在OP中的病理生理学意义尚不清楚。这项研究的目的是探讨脂质代谢重编程在OP中的作用。专注于炎症和纤维化。
方法:全面的多组学分析方法,包括单细胞RNA测序,铯细胞辅助空间转录组学,蛋白质组学,代谢组学和质谱,被用来分析组织。利用OP小鼠模型并在巨噬细胞中研究分子机制。
结果:结果显示OP与脂质代谢重编程之间存在显著关联,以与脂质代谢相关的几个基因的异常表达为特征,CD36、SCD1和CES1主要在巨噬细胞中。肺泡巨噬细胞CD36缺乏,导致线粒体中积累的C16/24神经酰胺的表达增加,导致线粒体自噬或线粒体功能障碍。OP中肺泡巨噬细胞的数量显著减少,这可能是由于在OP中通过CD36下调涉及GSH/SLC3A2/GPX4的铁凋亡信号通路。此外,巨噬细胞分泌DPP7和FABP4影响上皮细胞纤维化。
结论:CD36通过调节脂质代谢抑制OP组织肺泡巨噬细胞SLC3A2/GPX4的铁凋亡途径,因此代表了CD36介导的新的抗铁凋亡和抗纤维化作用,至少在某种程度上,由神经酰胺。
结论:我们的发现揭示了组织性肺炎与脂质代谢重编程之间的显著关联,并将对理解患者组织性肺炎的机制做出重大贡献。
BACKGROUND: Organising pneumonia (OP) is one of the most common and lethal diseases in the category of interstitial pneumonia, along with lung cancer. Reprogramming of lipid metabolism is a newly recognized hallmark of many diseases including cancer, cardiovascular disorders, as well as liver fibrosis and sclerosis. Increased levels of ceramides composed of sphingosine and fatty acid, are implicated in the development of both acute and chronic lung diseases. However, their pathophysiological significance in OP is unclear. The aim of this study was to investigate the role of lipid metabolism reprogramming in OP, focusing on inflammation and fibrosis.
METHODS: Comprehensive multi-omics profiling approaches, including single-cell RNA sequencing, Visium CytAssist spatial transcriptomics, proteomics, metabolomics and mass spectrometry, were employed to analyze the tissues. OP mice model was utilized and molecular mechanisms were investigated in macrophages.
RESULTS: The results revealed a significant association between OP and lipid metabolism reprogramming, characterized by an abnormal expression of several genes related to lipid metabolism, including CD36, SCD1, and CES1 mainly in macrophages. CD36 deficiency in alveolar macrophages, led to an increased expression of C16/24 ceramides that accumulated in mitochondria, resulting in mitophagy or mitochondrial dysfunction. The number of alveolar macrophages in OP was significantly reduced, which was probably due to the ferroptosis signaling pathway involving GSH/SLC3A2/GPX4 through CD36 downregulation in OP. Furthermore, macrophage secretion of DPP7 and FABP4 influenced epithelial cell fibrosis.
CONCLUSIONS: CD36 inhibited the ferroptosis pathway involving SLC3A2/GPX4 in alveolar macrophages of OP tissue by regulating lipid metabolism, thus representing a new anti-ferroptosis and anti-fibrosis effect of CD36 mediated, at least in part, by ceramides.
CONCLUSIONS: Our findings reveal a significant association between organising pneumonia and lipid metabolism reprogramming and will make a substantial contribution to the understanding of the mechanism of organising pneumonia in patients.