■臂丛神经损伤(BPI),虽然罕见,通常会导致严重的发病率。干细胞被认为是BPI治疗方式之一,因为它们具有形成神经的再生潜力。尽管存在使用间充质干细胞作为BPI治疗之一的可能性,它仍然局限于动物研究。因此,本文系统评价间充质干细胞在臂丛神经损伤动物模型中的作用。
■本研究是对PROSPERO注册号为CRD4202128321的系统评价。文献检索采用关键词实验,动物,臂丛神经损伤,间充质干细胞植入,临床结果,电生理结果,和组织学结果。搜索在PubMed中进行,Scopus,和ScienceDirect数据库。使用SYRCLE的动物研究偏倚风险工具评估偏倚风险。描述获得的数据并进行深入分析。
■这项研究中包括四项研究,涉及183只来自不同物种的动物,即大鼠和兔。在用干细胞处理的组中,存在肌肉重量的增加和肌肉收缩的初始起效时间的缩短。电生理结果表明,间充质干细胞表现出比对照更高的CMAP振幅(复合肌肉动作电位)和更短的CMAP潜伏期,但不优于自体移植物。组织学结果显示轴突密度增加,轴突数,以及神经细胞和目标肌肉之间连接的形成。
■间充质干细胞植入臂丛神经损伤的动物显示其再生神经细胞的能力,临床证明,电生理学,和组织病理学结果。然而,这项系统的研究涉及来自不同物种的实验动物,因此结果无法统一,结论应该谨慎。
UNASSIGNED: Brachial plexus injuries (BPI), although rare, often results in significant morbidity. Stem cell was thought to be one of BPI treatment modalities because of their
nerve-forming regeneration potential. Although there is a possibility for the use of mesenchymal stem cells as one of BPI treatment, it is still limited on animal studies. Therefore, this systematic
review aimed to analyze the role of mesenchymal stem cells in
nerve regeneration in animal models of brachial plexus injury.
UNASSIGNED: This study is a systematic
review with PROSPERO registration number CRD4202128321. Literature searching was conducted using keywords experimental, animal, brachial plexus injury, mesenchymal stem cell implantation, clinical outcomes, electrophysiological outcomes, and histologic outcomes. Searches were performed in the PubMed, Scopus, and ScienceDirect databases. The risk of bias was assessed using SYRCLE\'s risk of bias tool for animal studies. The data obtained were described and in-depth analysis was performed.
UNASSIGNED: Four studies were included in this study involving 183 animals from different species those are rats and rabbits. There was an increase in muscle weight and shortened initial onset time of muscle contraction in the group treated with stem cells. Electrophysiological results showed that mesenchymal stem cells exhibited higher (Compound muscle action potential) CMAP amplitude and shorter CMAP latency than control but not better than autograft. Histological outcomes showed an increase in axon density, axon number, and the formation of connections between
nerve cells and target muscles.
UNASSIGNED: Mesenchymal stem cell implantation to animals with brachial plexus injury showed its ability to regenerate
nerve cells as evidenced by clinical, electrophysiological, and histopathological results. However, this systematic study involved experimental animals from various species so that the results cannot be uniformed, and conclusion should be drawn cautiously.