空肠弯曲杆菌,一种人畜共患的食源性病原体,是世界范围内导致人类急性细菌性胃肠炎的主要原因。生物膜是这种病原体生存和传播的重要储库,有助于其整体抗菌素耐药性。天然化合物,如精油,植物化学物质,多酚提取物,和D-氨基酸已被证明具有控制细菌形成的生物膜的潜力,包括弯曲杆菌。这项工作提出了使用空肠弯曲杆菌作为模型,在天然存在的抑制分子存在下评估和表征细菌生物膜形成的拟议指南。以下方案描述:i)生物膜形成抑制测定,旨在评估天然分子抑制生物膜形成的能力;ii)生物膜分散测定,评估天然存在的抑制分子根除已建立的生物膜的能力;iii)共聚焦激光扫描显微镜(CLSM),在用天然存在的抑制分子处理后评估生物膜中的细菌活力,并研究处理前后生物膜的结构外观(或结构)。
Campylobacter jejuni, a zoonotic foodborne pathogen, is the worldwide leading cause of acute human bacterial gastroenteritis. Biofilms are a significant reservoir for survival and transmission of this pathogen, contributing to its overall antimicrobial resistance. Natural compounds such as essential oils, phytochemicals, polyphenolic extracts, and D-amino acids have been shown to have the potential to control biofilms formed by bacteria, including Campylobacter spp. This work presents a proposed
guideline for assessing and characterizing bacterial biofilm formation in the presence of naturally occurring inhibitory molecules using C. jejuni as a model. The following protocols describe: i) biofilm formation inhibition assay, designed to assess the ability of naturally occurring molecules to inhibit the formation of biofilms; ii) biofilm dispersal assay, to assess the ability of naturally occurring inhibitory molecules to eradicate established biofilms; iii) confocal laser scanning microscopy (CLSM), to evaluate bacterial viability in biofilms after treatment with naturally occurring inhibitory molecules and to study the structured appearance (or architecture) of biofilm before and after treatment.