背景:单鳞茎大蒜提取物(SBGE)比普通大蒜含有更多的活性化合物,但是它不稳定,在消化道中容易降解。SBGE有望受到微囊化壳聚糖-藻酸盐(MCA)的保护。
目的:本研究旨在表征和评估抗氧化活性,血液相容性,和MCA-SBGE对3T3-L1细胞的毒性。
方法:研究程序包括提取单茎大蒜,MCA-SBGE的制备,粒度分析仪(PSA),FTIR分析,DPPH测定,血液相容性试验,和MTT测定。
结果:MCA-SGBE的平均尺寸为423.7±2.8nm,多分散指数(PdI)为0.446±0.022,ζ电位为-24.5±0.4mV。MCA-SGBE为球形,直径范围为0.65-0.9µm。在封装后的SBGE中发现吸收和官能团的添加发生了变化。MCA-SBGE,浓度为24x103ppm,比SBGE有更高的抗氧化剂。血液相容性测试显示MCA-SBGE的溶血低于SBGE。MCA-SBGE对3T3-L1细胞没有毒性,在所有浓度下细胞活力百分比高于100%。
结论:MCA-SBGE表征具有均匀PdI值的微粒标准,颗粒稳定性低,和球形形态。结果表明,SBGE和MCA-SBGE均为非溶血性,与红细胞相容,对3T3-L1细胞无毒。
BACKGROUND: Single-bulb garlic extract (SBGE) contains more active compounds than regular garlic, but it is unstable and easily degraded in the digestive tract. SBGE is expected to be protected by
microencapsulation chitosan-alginate (MCA).
OBJECTIVE: The present
study aimed to characterize and assess the antioxidant activity, hemocompatibility, and toxicity of MCA-SBGE in 3T3-L1 cells.
METHODS: The research procedures consist of extraction of single bulb garlic, preparation of MCASBGE, Particle Size Analyzer (PSA), FTIR analysis, DPPH assay, hemocompatibility test, and MTT assay.
RESULTS: The average size of MCA-SGBE was 423.7 ± 2.8 nm, the polydispersity index (PdI) was 0.446 ± 0.022, and the zeta potential was -24.5 ± 0.4 mV. MCA-SGBE was spherical with a diameter range of 0.65-0.9 μm. A shift in absorption and addition of functional groups was found in SBGE after encapsulation. MCA-SBGE, at a concentration of 24 x 103 ppm, has higher antioxidants than SBGE. The hemocompatibility test shows the hemolysis of MCA-SBGE lower than SBGE. MCA-SBGE was not toxic to 3T3-L1 cells with cell viability percentage above 100% at all concentrations.
CONCLUSIONS: MCA-SBGE characterization has microparticle criteria with homogeneous PdI values, low particle stability, and spherical morphology. The results showed that SBGE and MCA-SBGE are nonhemolytic, compatible with red blood cells, and non-toxic to 3T3-L1 cells.