■绿茶提取物(GTE)的抗炎作用已在哮喘小鼠中得到证实,然而,药理机制尚未完全阐明。
■为了研究GTE在哮喘中的治疗效果并确定具体途径,通过卵清蛋白(OVA)致敏和攻击4周建立小鼠过敏性哮喘模型,使用GTE和地塞米松(DEX)口服治疗。炎症细胞计数,细胞因子,OVA特异性IgE,气道高反应性,并评估了肺中的抗氧化标志物。此外,进行肺组织病理学分析和蛋白质印迹.体外,我们通过用脂多糖刺激人气道上皮细胞系NCI-H292建立模型,并用GTE和丝裂原活化蛋白激酶(MAPKs)抑制剂处理。
■与OVA组相比,GTE100和GTE400组显示出气道高反应性和支气管肺泡灌洗液(BALF)中炎性细胞数量的降低。GTE治疗也降低了白细胞介素(IL)-13,IL-5和IL-4水平,与OVA组相比,血清中OVA特异性免疫球蛋白E水平。GTE治疗减少了OVA诱导的粘液分泌和气道炎症。此外,GTE抑制氧化应激,和MAPK的磷酸化,这通常发生在暴露于OVA之后。GTE给药还降低了基质金属蛋白酶-9活性和蛋白质水平。
■GTE有效抑制OVA吸入诱导的哮喘性呼吸道炎症和粘液过度产生。这些结果表明GTE具有用于治疗哮喘的潜力。
UNASSIGNED: The anti-inflammatory effect of green tea extract (GTE) has been confirmed in asthmatic mice, however, the pharmacological mechanism is not fully elucidated.
UNASSIGNED: To investigate the therapeutic efficacy of GTE in asthma and identify specific pathways, murine model of allergic asthma was established by ovalbumin (OVA) sensitization and the challenge for 4 weeks, with oral treatment using GTE and dexamethasone (DEX). Inflammatory cell counts, cytokines, OVA-specific IgE, airway hyperreactivity, and antioxidant markers in the lung were evaluated. Also, pulmonary histopathological analysis and western blotting were performed. In vitro, we established the model by stimulating the human airway epithelial cell line NCI-H292 using lipopolysaccharide, and treating with GTE and mitogen-activated protein kinases (MAPKs) inhibitors.
UNASSIGNED: The GTE100 and GTE400 groups showed a decrease in airway hyperresponsiveness and the number of inflammatory cells in the bronchoalveolar lavage fluid (BALF) compared to the OVA group. GTE treatment also reduced interleukin (IL)-13, IL-5, and IL-4 levels in the BALF, and OVA-specific immunoglobulin E levels in the serum compared to those in the OVA group. GTE treatment decreased OVA-induced mucus secretion and airway inflammation. In addition, GTE suppressed the oxidative stress, and phosphorylation of MAPKs, which generally occurs after exposure to OVA. GTE administration also reduced matrix metalloproteinase-9 activity and protein levels.
UNASSIGNED: GTE effectively inhibited asthmatic respiratory inflammation and mucus hyperproduction induced by OVA inhalation. These results suggest that GTE has the potential to be used for the treatment of asthma.