Abstract：
OBJECTIVE: The up-regulation of matrix metalloproteinase-9 (MMP-9) expression is a characteristic feature observed across various malignancies, including nasopharyngeal carcinoma (NPC). Nevertheless, the influence of MMP-9 genotype in the context of NPC remains underexplored. This study examined the implications of MMP-9 promoter rs3918242 genotypes on the susceptibility to NPC in Taiwan.
METHODS: In a cohort comprising 208 NPC cases and 416 healthy controls, genotyping of MMP-9 rs3918242 was conducted utilizing polymerase chain reaction-restriction fragment length polymorphism methodology.
RESULTS: Individuals harbouring the variant CT or TT genotype of MMP-9 rs3918242 did not demonstrate a discernible alteration in NPC risk when compared to wild-type CC carriers [odds ratio (OR)=0.83 and 0.79, with 95% confidence intervals (95%CI)=0.56-1.24 and 0.27-2.29; p=0.4205 and 0.8675, respectively]. Moreover, the presence of the variant T allele did not confer a modified risk of NPC (OR=0.84, 95%CI=0.60-1.19, p=0.3761). Intriguingly, a protective effect associated with the MMP-9 rs3918242 CT genotype against NPC risk was discerned among individuals abstaining from betel quid chewing behaviour (OR=0.51, 95%CI=0.30-0.87, p=0.0166). Notably, no significant association was established between the MMP-9 rs3918242 CT or TT genotype and NPC risk among individuals with or without smoking or alcohol consumption habits.
CONCLUSIONS: Presence of the variant CT or TT genotype at MMP-9 rs3918242 did not appear to substantially contribute to an elevated risk of NPC. Notably, a protective effect against NPC risk was observed in individuals carrying the CT genotype, particularly in those abstaining from betel quid chewing.
METHODS: In a cohort comprising 208 NPC cases and 416 healthy controls, genotyping of MMP-9 rs3918242 was conducted utilizing polymerase chain reaction-restriction fragment length polymorphism methodology.
RESULTS: Individuals harbouring the variant CT or TT genotype of MMP-9 rs3918242 did not demonstrate a discernible alteration in NPC risk when compared to wild-type CC carriers [odds ratio (OR)=0.83 and 0.79, with 95% confidence intervals (95%CI)=0.56-1.24 and 0.27-2.29; p=0.4205 and 0.8675, respectively]. Moreover, the presence of the variant T allele did not confer a modified risk of NPC (OR=0.84, 95%CI=0.60-1.19, p=0.3761). Intriguingly, a protective effect associated with the MMP-9 rs3918242 CT genotype against NPC risk was discerned among individuals abstaining from betel quid chewing behaviour (OR=0.51, 95%CI=0.30-0.87, p=0.0166). Notably, no significant association was established between the MMP-9 rs3918242 CT or TT genotype and NPC risk among individuals with or without smoking or alcohol consumption habits.
CONCLUSIONS: Presence of the variant CT or TT genotype at MMP-9 rs3918242 did not appear to substantially contribute to an elevated risk of NPC. Notably, a protective effect against NPC risk was observed in individuals carrying the CT genotype, particularly in those abstaining from betel quid chewing.
摘要：
目的：基质金属蛋白酶-9（MMP-9）表达上调是在各种恶性肿瘤中观察到的特征，包括鼻咽癌（NPC）。然而，MMP-9基因型在鼻咽癌中的影响仍未得到充分研究。这项研究探讨了MMP-9启动子rs3918242基因型对台湾NPC易感性的影响。
方法：在包括208例NPC和416例健康对照的队列中，采用聚合酶链反应-限制性片段长度多态性方法对MMP-9rs3918242进行基因分型。
结果：与野生型CC携带者相比，携带MMP-9rs3918242的变异CT或TT基因型的个体在NPC风险方面没有明显变化[比值比（OR）=0.83和0.79，95％置信区间（95CI）=0.56-1.24和0.27-2.29；p=0.4205和0.8675]。此外，变异T等位基因的存在并未改变NPC的风险(OR=0.84,95CI=0.60~1.19,p=0.3761).有趣的是，与MMP-9rs3918242CT基因型对NPC风险相关的保护作用在个体中被发现,个体放弃了槟榔咀嚼行为(OR=0.51,95CI=0.30-0.87,p=0.0166).值得注意的是,在有或没有吸烟或饮酒习惯的个体中,MMP-9rs3918242CT或TT基因型与NPC风险之间无显著关联.
结论：在MMP-9rs3918242中存在变异的CT或TT基因型似乎并没有实质上导致NPC风险升高。值得注意的是,在携带CT基因型的个体中观察到对NPC风险的保护作用，尤其是那些放弃嚼槟榔的人。
方法：在包括208例NPC和416例健康对照的队列中，采用聚合酶链反应-限制性片段长度多态性方法对MMP-9rs3918242进行基因分型。
结果：与野生型CC携带者相比，携带MMP-9rs3918242的变异CT或TT基因型的个体在NPC风险方面没有明显变化[比值比（OR）=0.83和0.79，95％置信区间（95CI）=0.56-1.24和0.27-2.29；p=0.4205和0.8675]。此外，变异T等位基因的存在并未改变NPC的风险(OR=0.84,95CI=0.60~1.19,p=0.3761).有趣的是，与MMP-9rs3918242CT基因型对NPC风险相关的保护作用在个体中被发现,个体放弃了槟榔咀嚼行为(OR=0.51,95CI=0.30-0.87,p=0.0166).值得注意的是,在有或没有吸烟或饮酒习惯的个体中,MMP-9rs3918242CT或TT基因型与NPC风险之间无显著关联.
结论：在MMP-9rs3918242中存在变异的CT或TT基因型似乎并没有实质上导致NPC风险升高。值得注意的是,在携带CT基因型的个体中观察到对NPC风险的保护作用，尤其是那些放弃嚼槟榔的人。
