基因簇正在成为基因鉴定的有希望的工具。该研究揭示了基因的目的基因组分布朝向完整代谢途径/表型的更高遗传率,因此,更高的健身。共同表达的共同本地化,共同互动,功能相关的基因被发现是人类全基因组趋势,鼠标,金鹰,稻米鱼,果蝇,花生,和拟南芥。如预期,分析验证了共定位事件的共隔离。共同定位事件的可能性与基因座间距离之间呈负相关。还发现基因组块的进化沿染色体臂收敛且均匀。因此,建议将负责相邻代谢反应的基因组块用于鉴定候选基因和解释细胞功能。作为一个案例故事,根据Slc25A44的基因组局部信息,提出了一种能量代谢和次生代谢产物的功能。Slc25A44被进一步表征为必需的管家基因,该基因一直处于进化净化压力下,属于SLC25s的系统发育ETC进化枝。途径富集将Slc25A44s映射到能量代谢。花生和人Slc25A44s在卵母细胞和酿酒酵母菌株中的表达证实了次级代谢产物和泛醌的常见前体的运输。这些结果表明,SLC25A44是具有生物技术应用的线粒体-ER-核区转运蛋白。最后,发现在运输腔的胞浆表面上保守的三个氨基酸特征对于SLC25s的合理工程很重要。
Gene clusters are becoming promising tools for gene identification. The study reveals the purposive genomic distribution of genes toward higher inheritance rates of intact metabolic pathways/phenotypes and, thereby, higher fitness. The co-localization of co-expressed, co-interacting, and functionally related genes was found as genome-wide trends in humans, mouse, golden eagle, rice fish, Drosophila, peanut, and Arabidopsis. As anticipated, the analyses verified the co-segregation of co-localized events. A negative correlation was notable between the likelihood of co-localization events and the inter-loci distances. The evolution of genomic blocks was also found convergent and uniform along the chromosomal arms. Calling a genomic block responsible for adjacent metabolic reactions is therefore recommended for identification of candidate genes and interpretation of cellular functions. As a
case story, a function in the metabolism of energy and secondary metabolites was proposed for Slc25A44, based on its genomic local information. Slc25A44 was further characterized as an essential housekeeping gene which has been under evolutionary purifying pressure and belongs to the phylogenetic ETC-clade of SLC25s. Pathway enrichment mapped the Slc25A44s to the energy metabolism. The expression of peanut and human Slc25A44s in oocytes and Saccharomyces cerevisiae strains confirmed the transport of common precursors for secondary metabolites and ubiquinone. These results suggest that SLC25A44 is a mitochondrion-ER-nucleus zone transporter with biotechnological applications. Finally, a conserved three-amino acid signature on the cytosolic face of transport cavity was found important for rational engineering of SLC25s.