TetR家族转录调节因子(TFRs)广泛分布于细菌和古细菌中,首次发现的TFR被证实能控制四环素外排泵在大肠杆菌中的表达。TFR可以结合DNA和配体。小分子配体可以诱导TFRs的构象变化,抑制或促进TFR以控制目标基因表达。目前,TFRs有各种各样的配体,包括碳水化合物,蛋白质,脂肪酸及其衍生物,金属离子,等等。由于配体的多样性,TFR调节广泛的生理过程,从基本的碳代谢和氮代谢到群体感应和抗生素生物合成。根据我们实验室的最新研究和文献,本文综述了TFRs配体在初级和次级代谢中的调控机制,以及TFRs配体在基因途径开发和抗生素生物合成激活中的应用。
TetR family transcriptional regulators (TFRs) are widely distributed in bacteria and archaea, and the first discovered TFR was confirmed to control the expression of tetracycline efflux pump in Escherichia coli. TFRs can bind DNAs and ligands. Small molecule ligands can induce conformational changes of TFRs, inhibiting or promoting TFRs to control target gene expression. Currently, TFRs have a wide variety of ligands, including carbohydrates, proteins, fatty acids and their derivatives, metal ions, and so on. Due to the diversity of ligands, TFRs regulate a wide range of physiological processes, from basic carbon metabolism and nitrogen metabolism to quorum sensing and antibiotic biosynthesis. On the basis of the recent studies in our laboratory and the literature, we
review here the regulatory mechanism mediated by ligands of TFRs in primary and secondary metabolism, as well as the application of ligands for TFRs in the development of gene route and the activation of antibiotic biosynthesis.