GLI基因介导的Hedgehog(Hh)信号通路在脑癌的发展和生长中起着重要作用,包括多形性胶质母细胞瘤(GBM),低级别的神经胶质瘤(LGG),和髓母细胞瘤(MB)。GLI2和GLI3基因表达水平在这些患者存活率较差的癌症中极度增强。此外,GLI基因与干性相关因子SOX2,SOX9,POU5F1和NANOG相关,它们是脑癌干细胞(CSC)进展的驱动因子。找到新的方法来对抗这种致命的恶性肿瘤和CSC是至关重要的。使用计算机模拟方法,我们的研究探讨了GLI基因(GLI1,GLI2和GLI3)的作用,SonicHedgehog(SHH)信号通路的初级转录因子,在GBM中,LGG,MB,和胶质母细胞瘤干细胞样细胞(GSC)。此外,我们发现血管生成相关基因VEGFA,代谢基因ENO1,ENO2和多能性相关基因SOX2,SOX9,NANOG,POU5F1与GLI基因,提示它们在脑肿瘤发生和进展中的作用。我们还研究了他们关于脑肿瘤发展的转录网络和功能类别富集分析,以找到针对脑癌及其干细胞的更好的治疗策略。
UNASSIGNED:在线版本包含补充材料,可在10.1007/s13205-022-03419-5获得。
GLI gene-mediated hedgehog (Hh) signaling pathway plays a substantial role in brain cancer development and growth including glioblastoma multiforme (GBM), lower-grade glioma (LGG), and medulloblastoma (MB). GLI2 and
GLI3 gene expression levels are extremely enhanced in these cancers with poor patient survival. Moreover, GLI genes are correlated with stemness-related factors SOX2, SOX9, POU5F1, and NANOG that work as the driving factors for brain cancer stem cells (CSCs) progression. It\'s critical to find new ways to combat this deadly malignancy and CSCs. Using in silico approaches, our study explored the role of GLI genes (GLI1, GLI2, and
GLI3), the primary transcription factors of the sonic hedgehog (SHH) signaling pathway, in GBM, LGG, MB, and glioblastoma stem-like cells (GSCs). Additionally, we found strong association of angiogenic-related gene VEGFA, metabolic genes ENO1, ENO2, and pluripotency-related genes SOX2, SOX9, NANOG, POU5F1 with GLI genes, suggesting their role in brain tumor initiation and progression. We also studied their transcriptional network and functional category enrichment analysis about brain tumor development to find a better therapeutic strategy against brain cancer and their stem cells.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-022-03419-5.