微生物多样性通常与环境条件相关。这项调查是一个案例研究,以弥补缺乏对生化相关性的知识,生理,以及在沙特阿拉伯王国环境条件下玉米种子传播病原真菌分布的分子属性,以帮助预测任何破坏性流行病。使用标准湿吸墨纸(SMB)检测到属于24属的41种真菌,深层冷冻吸墨纸(DFB),和琼脂平板(AP)技术。SMB在检测种子传播的分枝杆菌的最大数量(36种)方面表现优异。致病性测定显示,在使用的18种种子传播真菌病原体中,12个分离株引起高百分比的腐烂种子和幼苗死亡症状,使用内部转录的间隔序列进行分子鉴定。两种弯孢菌。KSA首次报道了玉米Sarocladium。在不同的环境设置下,菌株显示出各种酶活性和氨基酸谱。温度和湿度是影响真菌致病性的环境变量。最高的致病性与苏氨酸的存在和浓度相关,丙氨酸,谷氨酸,天冬氨酸,和蛋白质。这项研究的结论是在KSA中发现了四种新的植物病原体,进一步,证明了所调查变量之间的显著相关性。然而,鼓励更多的研究包括植物病原体的其他生理特性,例如产毒活动,以及将真菌生物多样性研究扩展到其他植物。
Microbiodiversity is usually correlated with environmental conditions. This investigation is a
case study to cover the lack of knowledge on the correlation of biochemical, physiological, and molecular attributes with the distribution of seed-borne pathogenic fungi of maize under the environmental conditions of the Kingdom of Saudi Arabia to help forecast any destructive epidemics. Forty-one fungal species belonging to 24 genera were detected using standard moist blotter (SMB), deep freezing blotter (DFB), and agar plate (AP) techniques. SMB was superior in detecting the maximum numbers (36 species) of seed-borne mycoflora. The pathogenicity assay revealed that, among 18 seed-borne fungal pathogens used, 12 isolates caused high percentages of rotted seeds and seedling mortality symptoms, which were identified molecularly using an internal transcribed spacer sequence. Two Curvularia spp. and Sarocladium zeae were reported for the first time in KSA. The strains showed various enzymatic activities and amino acid profiles under different environmental setups. Temperature and humidity were the environmental variables influencing the fungal pathogenicity. The highest pathogenicity was correlated with the presence and concentration of threonine, alanine, glutamic, aspartic acids, and protein. The study concluded with the discovery of four new phytopathogens in KSA and, further, evidenced a marked correlation among the investigated variables. Nevertheless, more studies are encouraged to include additional physiological properties of the phytopathogens, such as toxigenic activity, as well as extend the fungal biodiversity study to other plants.