■TrinakantamaniPishti(TMP)是一种强心剂(Hridya),厌食症(RaktaStambhaka),经常用于各种出血性疾病如腹泻(Raktatisaara)的收敛(Kashaya)制剂,Raktarsha(出血桩),和月经过多的疾病(Atyartava)。尽管如此,没有关于其表征的公开数据。
■使用复杂的仪器技术为原始和加工的TMP生成指纹,以评估TMP的抗微生物活性。
使用标准参考方法制备TMP的三个样品。通过傅里叶变换红外光谱(FTIR)对TMP进行表征,能量色散X射线分析(EDEX)与扫描电子显微镜,粉末X射线衍射(XRD)。通过孔扩散法进行抗菌活性。
■通过扫描电子显微镜分析显示,原始样品和TMP中的最大粒径<5μm和<3μm,分别。TMP中的最小粒度为1至2μm和701nm。EDEX分析显示碳和氧是主要成分,而Na,Mg,Ca,Si,Fe,S存在于痕迹中。XRD图表明药物的无定形性质,而FTIR分析显示存在官能团如O-H,CO2,C=O,C-N,N-H.重金属,微生物总数,和微生物限度测试被发现在允许的限度内。抗微生物研究对测试病原体金黄色葡萄球菌,大肠杆菌,铜绿假单胞菌和鼠伤寒沙门氏菌未显示TMP的任何作用。
■EDEX研究的结果表明,Pishti样品具有较小的粒径,即比原始的701nm,即,1-2μm,这可能会使药物吸收到体内。样品中的所有重金属均在允许范围内。碳,氢和氧是药物的主要元素,这证实了与琥珀的相似性,由于目前的工作是首次发表的关于TMP的表征和抗微生物研究的文献,结果可以被认为是使用上述参考方法制备的药物的指纹。
UNASSIGNED: Trinakantamani Pishti (TMP) is a cardio-tonic (Hridya), styptic (Rakta Stambhaka), astringent (Kashaya) formulation frequently used in varieties of bleeding disorders such as bloody diarrhea (Raktatisaara), Raktarsha (bleeding piles), and disorders of excessive menstruation (Atyartava). Still, no published data is available regarding its characterization.
UNASSIGNED: To generate a fingerprint for raw and processed TMP using sophisticated instrumental techniques to assess antimicrobial activity of TMP.
UNASSIGNED: Three samples of TMP were prepared using the standard reference method. Characterization of TMP was carried out by Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray analysis (EDEX) with scanning electron microscopy, powder X-ray diffraction (XRD). Antibacterial activity was carried out by the well-diffusion method.
UNASSIGNED: Analysis by scanning electron microscope revealed maximum particle size <5 μm and <3 μm in the raw sample and TMP, respectively. Minimum particle size in TMP ranges from 1 to 2 μm and 701 nm. EDEX analysis shows carbon and oxygen as major constituents while Na, Mg, Ca, Si, Fe, and S were present in traces. XRD pattern indicates the amorphous nature of the drug, while FTIR analysis reveals the presence of functional groups such as O-H, CO2, C = O, C-N, N-H. Heavy metals, total microbial count, and microbial limit test were found to be under permissible limits. Anti-microbial
study against tested pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium did not show any effect of TMP.
UNASSIGNED: The results of EDEX
study showed that Pishti samples have the small particle size i.e., 701nm than the raw i.e., 1-2 μm, which may facillitate absobtion of drug into the body. All heavy metals in the samples were within the permissible limit. Carbon, hydrogen and oxygen are the chief elements of drug which confirms similarity to the Amber, Since the present work is the first published literature on characterization and anti-microbial
study on TMP, the outcome can be considered as fingerprint for the drug prepared using the mentioned reference method.