生物胺(BA)的生产,它们是鱼类和渔业产品质量和安全的标志,受收获技术的影响,处理,以及其他行动,包括在船上进行的行动。暗肉鱼(例如金枪鱼)是与组胺中毒最常见的鱼类。鱼类中最常见的BAs是组胺,酪胺,腐胺,还有尸体,当微生物将相应的游离氨基酸脱羧时产生。在这项研究中,开发并验证了一种快速且经济有效的HILIC-MS/MS方法,用于测定腐胺,尸体,金枪鱼样品中的组胺和酪胺。使用溶剂混合物MeOH/H2O(50/50,v/v)进行简单的样品制备程序,用于蛋白沉淀和分析物提取的0.1%乙酸。日内和日间准确性,表示为%回收率(%R),范围从88.0%(Cad)到102.7%(Tyr),从85.0%(Cad)到99.8%(Tyr),分别。日内和日间精度,表示为%相对标准偏差(%RSD),范围从0.4%(Tyr,看跌期权)至3.3%(His),从0.7%(Tyr)至5.0%(Cad),分别。检测限值(LOD)和定量限值(LOQ)从0.0009到0.0940mg/kg,从0.0030mg/kg到0.3100mg/kg,分别,取决于分析物。关于食品中与生物胺有关的潜在毒性作用,在这项研究中检查的样本显示没有风险。所提出的方法是对鱼产品中的BA进行常规分析的重要分析工具。
The production of biogenic amines (BAs), which are markers of both quality and safety in fish and fishery products, is influenced by the harvesting technique, handling, and other operations including those carried out on board the vessel. Scombroid dark-meat fish (e.g. tuna) are the fish species most frequently linked to histamine poisoning. The most commonly found BAs in fish are histamine, tyramine, putrescine, and cadaverine, which are produced when microbes decarboxylate the corresponding free amino acids. In this study, a rapid and cost-effective HILIC-MS/MS method was developed and validated for the determination of putrescine, cadaverine, histamine and tyramine in tuna samples. A simple sample preparation procedure was followed using the solvent mixture MeOH/H2O (50/50, v/v), 0.1 % acetic acid for protein precipitation and analyte extraction. Intra- and inter-day accuracy, expressed as %Recovery (%R), ranged from 88.0 % (Cad) to 102.7 % (Tyr) and from 85.0 % (Cad) to 99.8 % (Tyr), respectively. Intra- and inter-day precision, expressed as %Relative Standard Deviation (%RSD), ranged from 0.4 % (Tyr, Put) to 3.3 % (His) and from 0.7 % (Tyr) to 5.0 % (Cad), respectively. Limits of detection (LOD) and quantification (LOQ) varied from 0.0009 to 0.0940 mg/kg and from 0.0030 mg/kg to 0.3100 mg/kg, respectively, depending on the analyte. Regarding the potential toxic effects linked to biogenic amines in foods, samples examined in this study showed no risk. The proposed method is an important analytical tool for routine analysis of BAs in fish products.