Abstract：
Fishery products are one of the main human nutritional sources, and due to the consumption increase, the quality of the derived products may be modified, during catching, technological processing, and storage. Detection and identification of pathogenic and spoilage microorganisms in fishery products is needed because the first may be involved in human diseases, while the second is responsible of significant economic losses. In this sense, liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method and computational analysis of MS data are useful tools for characterizing and identifying different microorganisms and to develop promising strategies for food science investigations. Moreover, in the past decade, metaproteomic methodologies have progressed for the study of microorganisms isolated from their natural samples and independently of the culture restrictions. Metaproteomics enables assessment of proteins and pathways from individual members of the consortium. Metaproteomics can provide a detailed understanding of which organisms occupy specific metabolic niches, how they interact, and how they utilize nutrients, and these insights can be obtained directly from environmental samples.According to that, the sample preparation of the fishery product, the LC-ESI-MS/MS dedicated method, and the MS data analysis were described in the present chapter to obtain the metaproteomic analysis of the respective microbiomes or microbial communities.
摘要：
渔业产品是人类主要的营养来源之一,由于消费的增加，衍生产品的质量可能会改变，在捕捉过程中，技术处理,和存储。渔业产品中的病原微生物和腐败微生物的检测和鉴定是需要的，因为首先可能涉及人类疾病，而第二个是造成重大经济损失的原因。在这个意义上,液相色谱-电喷雾电离串联质谱（LC-ESI-MS/MS）方法和MS数据的计算分析是表征和鉴定不同微生物并为食品科学研究开发有前途的策略的有用工具。此外，在过去的十年里，元蛋白质组学方法在研究从其天然样品中分离出的微生物并且独立于培养物限制方面取得了进展。元蛋白质组学能够评估来自财团各个成员的蛋白质和途径。元蛋白质组学可以详细了解哪些生物体占据特定的代谢生态位，它们如何相互作用，以及它们如何利用营养，这些见解可以直接从环境样本中获得。据此,渔业产品的样品制备，LC-ESI-MS/MS专用方法，和MS数据分析在本章中进行了描述，以获得相应微生物群或微生物群落的元蛋白质组学分析。
