背景:非酒精性脂肪性肝病(NAFLD)与肥胖和糖尿病相关,可能会产生不同的游离脂肪酸(FFA)模式,但是对这种现象的理解是有限的。为此,我们评估了按体重分层的健康受试者和NAFLD患者的FFA概况,确定FFA对NAFLD的早期诊断有价值。
方法:健康和NAFLD的血清FFA谱(瘦,使用气相色谱-质谱(GC-MS)确定超重和肥胖)受试者,并使用单因素方差分析评估FFA模式的差异,同时使用接收器工作特征(ROC)和逻辑回归模型探索FFA对诊断NAFLD的意义。
结果:NAFLD患者与健康对照组(HC)相比,血清FFA谱明显更高(P<0.05)。虽然总FFA谱在瘦(2093.33±558.11μg/ml)和超重(2420.81±555.18μg/ml)NAFLD患者之间没有显着差异,与HC相比,肥胖NAFLD(2739.01±810.35μg/ml)呈现最显著升高(P<0.05)的总FFA谱.在四种FFA中;肉豆蔻酸(14:0),棕榈油酸(16:1),γ-亚麻酸(γ-18:3)和顺式-7,10,13,16,19-二十二碳五烯酸(22:5),在ROC分析中选择,考虑到他们的高Youden指数和AUC,只有14:0;5.58(1.37,22.76)和16:1;4.36(1.34,14.13)的奇数比具有统计学意义。
结论:我们的研究结果表明14:0和16:1对NAFLD的早期诊断是有希望的。
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) given its association with obesity and diabetes may perhaps exert distinct free fatty acids (FFA) pattern, but the understanding of this phenomenon is limited. To this effect, we evaluated FFA profiles among healthy subjects and NAFLD patients stratified by body weight, to identify FFA valuable for early diagnosis of NAFLD.
METHODS: Serum FFA profiles of healthy and NAFLD (lean, overweight and obese) subjects was determined using gas chromatography-mass spectrometry (GC-MS) and distinctions in FFA patterns were evaluated using one-way ANOVA while Receiver operating characteristics (ROC) and logistic regression models were used to explore FFA significant for diagnosing NAFLD.
RESULTS: NAFLD patients presented significantly higher (P < 0.05) serum FFA profiles compared to healthy controls (HC). While total FFA profiles were insignificantly different between lean (2093.33 ± 558.11 μg/ml) and overweight (2420.81 ± 555.18 μg/ml) NAFLD patients, obese NAFLD (2739.01 ± 810.35 μg/ml) presented most significantly elevated (P < 0.05) total FFA profiles compared with HC. Of the four FFA; myristic acid (14:0), palmitoleic acid (16:1), γ-linolenic acid (γ-18:3) and cis-7,10,13,16,19-docosapentaenoic acid (22:5), selected in ROC analysis given their high Youden\'s index and AUC, only 14:0; 5.58(1.37, 22.76) and 16:1; 4.36(1.34, 14.13) had statistical significant odd ratios.
CONCLUSIONS: Our findings suggest 14:0 and 16:1 are promising for early diagnosis of NAFLD.