Staphylococcus aureus has posed a huge threat to human health and the economy. Oleuropein has antibacterial activities against various microorganisms but research on its effect on the S. aureus biofilm is limited. This research aimed to estimate the antibiofilm activities of oleuropein against S. aureus. The results suggest that the minimum inhibitory concentration of oleuropein against S. aureus ATCC 25923 was 3 mg/mL. The biomass of biofilms formed on the microplates and coverslips and the viability of bacteria were significantly reduced after the treatment with oleuropein. The scanning electron microscopy observation results indicated that the stacking thickness and density of the biofilm decreased when S. aureus was exposed to oleuropein. It had a bactericidal effect on biofilm bacteria and removed polysaccharides and proteins from mature biofilms. The effects of oleuropein on the biofilm could be explained by a reduction in bacterial secretion of extracellular polymeric substances and a change in bacterial surface hydrophobicity. Based on the above findings, oleuropein has the potential to be used against food pollution caused by S. aureus biofilms.

To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S. sanguis, S. gordonii, and L. acidophilus were co-cultured to form 24 h, 48 h, and 72 h biofilms. The experimental groups were treated with bovine trypsin for 30 s, 1 min, and 3 min. Morphological observation and quantitative analysis of extracellular polymeric substances (EPS), live bacteria, and dead bacteria were conducted using the confocal laser scanning microscope (CLSM). The morphological changes of EPS and bacteria were also observed using a scanning electron microscope (SEM). When biofilm was treated for 1 min, the minimal inhibitory concentration (MIC) of bovine trypsin to reduce EPS was 0.5 mg/mL in 24 h and 48 h biofilms, and the MIC of bovine trypsin was 2.5 mg/mL in 72 h biofilms (P < 0.05). When biofilm was treated for 3 min, the MIC of bovine trypsin to reduce EPS was 0.25 mg/mL in 24 h and 48 h biofilms, the MIC of bovine trypsin was 1 mg/mL in 72 h biofilm (P < 0.05). The ratio of live-to-dead bacteria in the treatment group was significantly lower than blank group in 24 h, 48 h, and 72 h multispecies biofilms (P < 0.05). Bovine trypsin can destroy multispecies biofilm structure, disperse biofilm and bacteria flora, and reduce the EPS and bacterial biomass in vitro, which are positively correlated with the application time and concentration.

This study employs a bibliography study method to evaluate 472 papers focused on Shewanella oneidensis biofilms. Biofilms, which are formed when microorganisms adhere to surfaces or interfaces, play a crucial role in various natural, engineered, and medical settings. Within biofilms, microorganisms are enclosed in extracellular polymeric substances (EPS), creating a stable working environment. This characteristic enhances the practicality of biofilm-based systems in natural bioreactors, as they are less susceptible to temperature and pH fluctuations compared to enzyme-based bioprocesses. Shewanella oneidensis, a nonpathogenic bacterium with the ability to transfer electrons, serves as an example of a species isolated from its environment that exhibits extensive biofilm applications. These applications, such as heavy metal removal, offer potential benefits for environmental engineering and human health. This paper presents a comprehensive examination and review of the biology and engineering aspects of Shewanella biofilms, providing valuable insights into their functionality.

To investigate the degradation effect of bovine trypsin on multispecies biofilm of periodontitis-related bacteria and to provide an experimental reference for exploring new methods for controlling biofilms of periodontitis-related microorganisms, the multispecies biofilm of periodontitis-related microorganisms was established. Standard strains of Porphyromonas gingivalis, Fusobacterium nucleatum subsp. polymorpha, Actinomyces viscosus, and Aggregatibacter actinomycetemcomitans were co-cultured to form the biofilm. The experimental groups were treated with bovine trypsin, distilled water was applied as the blank control group, and phosphate saline buffer (pH = 7.4) as the negative control group. Morphological observation and quantitative analysis of extracellular polymeric substances (EPS), live bacteria, and dead bacteria were conducted using a laser confocal microscope. The morphological changes of EPS and bacteria were also observed using a scanning electron microscope. The results of morphological observations of modeling were as follows. EPS aggregated as agglomerates, and bacteria flora were wrapped by them, showing a three-dimensional network structure, and channel-like structures were inside the biofilm. Live bacteria were distributed on the surface of the EPS or embedded in them, dead bacteria aggregated between live flora and the bottom layer of biofilms. After being treated with bovine trypsin, the three-dimensional network structure and the channel-like structure disappeared, and the EPS and live and dead bacteria decreased. Quantitative analysis results are as follows. When biofilm was treated for 30 s, 1 min, and 3 min, the minimum effective concentrations of bovine trypsin to reduce EPS were 2 mg/ml (P < 0.05), 0.5 mg/ml (P < 0.05), and 0.25 mg/ml (P < 0.05), respectively. The minimum effective concentrations of bovine trypsin to reduce the live or dead bacteria were 2 mg/ml (P < 0.05), 0.5 mg/ml (P < 0.05), and 0.5 mg/ml (P < 0.05), respectively. There was no significant difference in the ratio of live/dead bacteria after the biofilm was treated for 30 s with bovine trypsin at the concentration of 0.25, 0.5, 1, and 2 mg/ml (P > 0.05), and the minimum effective concentration to reduce the ratio of live bacteria/dead bacteria was 0.25 mg/ml (P < 0.05) after treatment for 1 min and 3 min. Therefore, bovine trypsin can destroy biofilm structure, disperse biofilm and bacteria flora, and reduce the EPS and bacterial biomass, which are positively correlated with the application time and concentration.

In this study, Fe2+ activated-PAA was developed as a novel technology to enhance sludge dewatering. The result showed that the filterability (CST0/CST) enhanced by 4.20 ± 0.14 times more than the control, and the SRF and bound water content decreased from 4.58 ± 0.07 × 1013 m/kg and 2.11 ± 0.28 g/g dry sludge to 9.47 ± 0.05 × 1012 m/kg and 1.27 ± 0.18 g/g dry sludge, respectively after the sludge was conditioned by 1.20 mM/g VSS Fe2+ and 1.20 mM/g VSS PAA. The dewatering performance, physicochemical properties, aggregation behaviors, and EPS fractions of sludge were compared before and after Fe2+/PAA and Fe2+/H2O2 conditionings. The results showed that Fe2+/PAA treatment was more competitive in enhancing dewaterability under neutral and alkaline conditions than Fe2+/H2O2 treatment but slightly weaker under acid conditions. Besides, it was found that the oxidation and re-flocculation behaviors were different in those two enhanced dewatering technologies due to the difference in the generated ROS. R-O was the primary radical in the Fe2+/PAA system, while OH was the major one in the Fe2+/H2O2 system. The mechanism analysis found that the Fe2+/PAA process caused harsher disintegration of sludge flocs, meaning more generation of fine particles. However, it exhibited less effect on reducing the energy barrier between sludge particles. Therefore, the Fe2+/PAA treated sludge presented weaker aggregation behaviors. The weaker aggregation was unfavorable for sludge dewatering because the weaker aggregated flocs were more easily fragmented, which hampered the consolidation of sludge cakes and removal of bound water. Moreover, loosely-bound extracellular polymeric substances, particularly tightly-bound extracellular polymeric substances, governed the sludge dewaterability.

Integrating hydrothermal treatment (HT) and advanced oxidation processes (AOP) was proved to be a promising approach for improving sludge dewaterability. In this study, the EPS valorization under elevated temperature and sulfate radical-based AOP were investigated to clarify the valorization of organic matter in different EPS layers and its effects on the sludge dewaterability. Results indicated that the organic matters in the inner layer of EPS decreased sharply with the elevated temperature, and released into the soluble EPS. Sulfate radical-based AOP significantly accelerated the degradation of organics and microbial cells lysis, especially in the presence of ZVI. The protein with the higher hydrophobicity was detected under the AOP enhanced HT. A better synergistic effect on sludge dewaterability was obtained by integrated the AOP at the initial hydrothermal stage. 3D-EEM and parallel factor analysis indicated that the protein and microbial by-product like substances in tightly bound EPS significantly affected the dewaterability.

Simultaneous removal of polycyclic aromatic hydrocarbons (PAHs) in the process of enhancement of sludge dewaterability via oxidation of hydroxyl radicals (•OH) and flocculation of Fe3+ by Fe2+-catalyzing O3 were investigated as a novel research focus. The results showed that capillary suction time (CST) and water content of dewatered sludge cake (Wc) were reduced from 57.9 s and 85.1% to 13.6 s and 69.65% under the optimum usage of 60 mg/g dry solids (DS) O3 and 80 mg/g DS FeSO4, respectively. The relevant dewatering mechanism of Fe2+-catalyzing O3 treatment was elucidated. It was found that extracellular polymeric substances-bound (EPS-bound) and intracellular water was dramatically released through destroying sludge cells and EPS gel-like structure by produced •OH. In addition, the results of X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR) and 13C NMR spectroscopy revealed that •OH oxidized and mineralized hydrophilic organic matters intensifying hydrophobicity of sludge surface. Moreover, Fe3+ generated by oxidation of Fe2+ agglomerated fragmented fine particles into large aggregates and decreased exposure of hydrophilic sites by neutralizing negative charge, which promoted water-solids separation. Meanwhile, sludge surface roughness was decreased which was determined by material type upright confocal laser microscope (CLM). As a consequence, •OH and Fe3+ were mainly responsible for enhancement of sludge dewaterability. Moreover, more than 40% of removal rate of PAHs was accomplished by Fe2+-catalyzed O3 treatment mitigating the environmental risks of PAHs spread.

Microbial-assisted antimonate [Sb(V)] reduction immobilizes this redox-sensitive metalloid in the subsurface. Most indigenous aerobes in antimony (Sb)-contaminated areas do not contain Sb(V)-reducing genes but can resist high levels of Sb(V) threat. Herein, to unravel the mechanisms of Sb(V) resistance by aerobes, we used Escherichia coli W3110 as a model aerobe and incubated it with 10 μM Sb(V). We found that strain W3110, without known Sb(V)-reducing genes, was able to reduce Sb(V) to Sb(III). Our transcriptome analysis and reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) results show that the Sb(V) threat at the 10 μM level had a negligible effect on the gene expression of strain W3110. In vitro incubation experiments further indicate that Sb(V) reduction was attributable to extracellular polymeric substances (EPS). Moreover, the three-dimensional excitation-emission matrix fluorescence spectroscopy reveals that the tryptophan-like components in EPS were involved in Sb(V) binding as evidenced by its weakened fluorescence intensity upon Sb(V) addition. The FTIR and XPS analyses indicate that hemiacetal and amide groups in EPS contributed to the reduction of Sb(V). Preculture with 10 μM Sb(V) did not exhibit a significant difference in Sb(V)-reducing capacity, suggesting that Sb(V) stress probably did not stimulate EPS secretion of W3110. Our results highlight the importance of EPS as the first line of defense against toxins, especially for those bacteria without such functional genes.

A large amount of waste activated sludge (WAS) and food waste (FW) are produced every year in China. Anaerobic co-digestion is considered to be an effective way to solve this problem. This study applied FW/WAS mixture as co-substrate to create different digestive environment, aiming to understand the mechanism of Fe3O4 particles in promoting AD performance. The results showed that the addition of Fe3O4 presented various performances when facing different digestive acidification stress brought by different mixing ratios of WAS and FW. Methanogenic pathways and microbial communities varied with substrates\' properties. For group A (WAS mono-digestion), the acetoclastic methanogens dominated, 20 mg/g VS (according to the iron element) Fe3O4 could promote methane production, while 200 mg/g VS Fe3O4 would inhibit microbial activity. The promoted methane production by Fe3O4 was attributable to the promotion of sludge hydrolysis. For group B (WAS: FW = 1:0.5, based on VS addition, similarly hereinafter), Fe3O4 triggered direct interspecific electron transfer (DIET) between bacteria and methanogens. For group C (WAS: FW = 1:1), the hydrogenotrophic methanogens dominated, bacteria excreted more non-conductive polysaccharides in EPS to resist unfavorable environment, thereby it prevented their contact with Fe3O4 particles. So, it was difficult for Fe3O4 to trigger DIET and promote the digestive performance of batch experiments in such condition.

The difficulties in dewatering waste-activated sludge (WAS) using mechanical devices have caused great problems in sludge transportation and disposal. Herein, coagulation and flocculation are combined with the use of a magnetic field as a clean and low-energy physical treatment method to enhance the dewaterability of municipal and citric acid-processing WAS. It is shown that the use of the magnetic field had a significant effect on the capillary suction time (CST) of municipal WAS but not on the specific resistance filtration (SRF) and CST of the citric acid WAS. The differences in the magnetic field effects were due to differences in the sludge properties. For municipal WAS, the particle size decreased, the zeta potential remained unchanged, and the viscosity decreased, whereas in the citric acid WAS, the particle size increased, the absolute value of the zeta potential decreased, and the viscosity increased. In addition, these effects were also confirmed with studies of the water state and micro-morphology analyses. It is shown that the acidification of the municipal WAS and coagulation of citric acid WAS were likely the reasons for the enhancement of their dewaterability, respectively. This study confirmed that the use of a magnetic field combined with coagulation/flocculation may serve as an effective sludge conditioning method; however, the treatment conditions may vary with the sludge type.