Extracellular polymeric substances (EPS) regulated by quorum sensing (QS) could directly mediate adhesion between microorganisms and form tight microbial aggregates. Besides, EPS have redox properties, which can facilitate electron transfer for promoting electroactive bacteria. Currently, the applications research on improving wastewater biological treatment performance based on QS regulated EPS have been widely reported, but reviews on the level of QS regulated EPS to enhance EPS function in microbial systems are still lacking. This work proposes the potential mechanisms of EPS synthesis by QS regulation from the viewpoint of material metabolism and energy metabolism, and summarizes the effects of QS on EPS synthesis. By synthesizing the role of QS in EPS regulation, we further point out the applications of QS-regulated EPS in wastewater biological treatment, which involve a series of aspects such as strengthening microbial colonization, mitigating membrane biofouling, improving the shock resistance of microbial metabolic systems, and strengthening the electron transfer capacity of microbial metabolic systems. According to this comprehensive review, future research on QS-regulated EPS should focus on the exploration of the micro-mechanisms, and economic regulation strategies for QS-regulated EPS should be developed, while the stability of QS-regulated EPS in long-term production experimental research should be further demonstrated.

The evolution of emerging contaminants (ECs) has caused greater requirements and challenges to the current biological wastewater treatment technology. As one of the most promising biological treatment technologies, the aerobic granular sludge (AGS) process also faces the challenge of ECs. This study summarizes the recent progress and characteristics of several representative ECs (persistent organic pollutants, endocrine disrupting chemicals, antibiotics, and microplastics) in AGS systems that have garnered widespread attention. Additionally, the biodegradation and adsorption mechanisms of ECs were discussed, and the interactions between various ECs and AGS was elucidated. The importance of extracellular polymeric substances for the stabilization of AGS and the removal of ECs is also discussed. Knowledge gaps and future research directions that may enable the practical application of AGS are highlighted. Overall, AGS processes show great application potential and this review provides guidance for the future implementation of AGS technology as well as elucidating the mechanism of its interaction with ECs.

Biofilms are an aggregation of microorganisms that have high resistance to antimicrobial agents. In the food industry, it has been widely studied that foodborne pathogens on both food surfaces and food-contact surfaces can form biofilms thereby threatening the safety of the food. In the natural environment, multi-species biofilms formed by more than two different microorganisms are abundant. In addition, the resistance of multi-species biofilms to antimicrobial agents is higher than that of mono-species biofilms. Therefore, studies to elucidate the mechanisms of multi-species biofilms formed by foodborne pathogens are still required in the food industry. In this review paper, we summarized the novel analytical methods studied to evaluate the mechanisms of multi-species biofilms formed by foodborne pathogens by dividing them into four categories: spatial distribution, bacterial interaction, extracellular polymeric substance production and quorum sensing analytical methods.

A mathematical correlation between biomass kinetic and membrane fouling can improve the understanding and spread of Membrane Bioreactor (MBR) technology, especially in solving the membrane fouling issues. On this behalf, this paper, produced by the International Water Association (IWA) Task Group on Membrane modelling and control, reviews the current state-of-the-art regarding the modelling of kinetic processes of biomass, focusing on modelling production and utilization of soluble microbial products (SMP) and extracellular polymeric substances (EPS). The key findings of this work show that the new conceptual approaches focus on the role of different bacterial groups in the formation and degradation of SMP/EPS. Even though several studies have been published regarding SMP modelling, there still needs to be more information due to the highly complicated SMP nature to facilitate the accurate modelling of membrane fouling. The EPS group has seldom been addressed in the literature, probably due to the knowledge deficiency concerning the triggers for production and degradation pathways in MBR systems, which require further efforts. Finally, the successful model applications showed that proper estimation of SMP and EPS by modelling approaches could optimise membrane fouling, which can influence the MBR energy consumption, operating costs, and greenhouse gas emissions.

Antibiotics, the most frequently prescribed drugs, have been widely applied to prevent or cure human and veterinary diseases and have undoubtedly led to massive releases into sewer networks and wastewater treatment systems, a hotspot where the occurrence and transformation of antibiotic resistance take place. Extracellular polymeric substances (EPS), biopolymers secreted via microbial activity, play an important role in cell adhesion, nutrient retention, and toxicity resistance. However, the potential roles of sludge EPS related to the resistance and removal of antibiotics are still unclear. This work summarizes the composition and physicochemical characteristics of state-of-the-art microbial EPS, highlights the critical role of EPS in antibiotics removal, evaluates their defense performances under different antibiotics exposures, and analyzes the typical factors that could affect the sorption and biotransformation behavior of antibiotics. Next, interactions between microbial EPS and antibiotic resistance genes are analyzed. Future perspectives, especially the engineering application of microbial EPS for antibiotics toxicity detection and defense, are also emphatically stressed.

The anammox-based process has been considered a promising biological nitrogen elimination method for the treatment of nitrogen-rich wastewater ever since its discovery 40 years ago. However, the slow growth rate of anammox bacteria and severe sludge washout result in a long startup period and limit its widespread industrial application. A membrane bioreactor (MBR) is considered an ideal reactor for the operation of the anammox-based process because the membranes allow for 100 % biomass retention. According to a systematic review of the literature, anammox-based MBR is becoming a research hotspot in the field of nitrogen wastewater treatment. The fundamental understanding of anammox-based MBR and its membrane fouling situation is essential for the development and application of anammox-based MBR. In this paper, the application of MBR in different kinds of anammox process are reviewed. The membrane fouling mechanism and strategies to control membrane fouling are also proposed. It is expected that this review will serve as an invaluable guide for future research and in the engineering applications of anammox-based MBR process.

A review of the characterization and functions of extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems is presented in this paper. EPS represent the complex high-molecular-weight mixture of polymers excreted by microorganisms generated from cell lysis as well as adsorbed inorganic and organic matter from wastewater. EPS exhibit a three-dimensional, gel-like, highly hydrated matrix that facilitates microbial attachment, embedding, and immobilization. EPS play multiple roles in containments removal, and the main components of EPS crucially influence the properties of microbial aggregates, such as adsorption ability, stability, and formation capacity. Moreover, EPS are important to sludge bioflocculation, settleability, and dewatering properties and could be used as carbon and energy sources in wastewater treatment. However, due to the complex structure of EPS, related knowledge is incomplete, and further research is necessary to understand fully the precise roles in biological treatment processes.

Biofilm formation is a serious threat in the meat industry, mainly since it aids food-borne pathogen survival. Biofilms are often difficult to eliminate, and it is essential to understand the best possible deployable measures to remove or inactivate biofilms. We systematically reviewed the published in vitro studies that investigated various methods for removing biofilms in red meat. Publicly available databases, including Google Scholar and PubMed, were queried for relevant studies. The search was restricted to articles published in the English language from 2010 to 2021. We mined a total of 394 studies, of which 12 articles were included in this review. In summary, the studies demonstrated the inhibitory effect of various methods, including the use of bacteriophages, dry heat, cold atmospheric pressure, ozone gas, oils, and acids, on red meat extract or red meat culture. This systematic review suggests that in addition to existing sanitation and antibiotic procedures, other methods, such as the use of phage cocktails and different oils as nanoparticles, yield positive outcomes and may be taken from the in vitro setting to industry with prior validation of the techniques.

Extracellular Polymeric Substances (EPS) existent in anaerobic sludge proves to be a barrier for sludge liquefaction and biomass lysis efficiency. Hence EPS deaggregation heightens the surface area for the subsequent pretreatment thereby uplifting the sludge disintegration and biomethanation rate. This review documents the role of EPS and its components which inhibits sludge hydrolysis and also the various phase separated pretreatment methods available with its disintegration mechanism to enhance the biomass lysis and methane production rate. It also illustrates the effects of phase separated pretreatment on the sludge disintegration rate which embodies two phases-floc disruption and cell lysis accompanied by their computation through biomethane potential assay and fermentation analysis comprehensively. Additionally, energy balance study and cost analysis requisite for successful implementation of a proposed phase separated pretreatment on a pilot scale level and their challenges are also reviewed. Overall this paper documents the potency of phase separated pretreatment for full scale approach.

Multi-species biofilms are ubiquitous worldwide and are a concern in the food industry. Multi-species biofilms have a higher resistance to antimicrobial therapies than mono-species biofilms. In addition, multi-species biofilms can cause severe foodborne diseases. To remove multi-species biofilms, controlling the formation process of extracellular polymeric substances (EPS) and quorum sensing (QS) effects is essential. EPS disruption, inhibition of QS, and disinfection have been utilized to remove multi-species biofilms. This review presents information on the formation and novel removal methods for multi-species biofilms.