细胞外囊泡(EV)穿梭蛋白,RNA,DNA,和对细胞间通讯至关重要的脂质。最近的调查结果强调,电动汽车,凭借他们的货物,也可能导致乳腺癌(BC)的生长和转移扩散。的确,电动汽车作为非侵入性癌症生物标志物正在获得极大的兴趣。然而,对恶性BC病变的电动汽车的生物学和物理性质知之甚少,对来自非恶性病变的电动汽车的了解更少,如乳腺纤维腺瘤(FAD),这是临床管理使用保守的方法。因此,对于这项试点研究,我们试图从良性乳腺病变中纯化和探索EV的蛋白质组学图谱,HER2+BCs,三阴性BCs(TNBC),和连续的BC细胞系(即,BT-549,MCF-10A,和MDA-MB-231),结合实验和半定量方法。值得注意的是,全蛋白质组分析显示,从FAD收获的电动汽车中有49种常见蛋白质,HER2+BCs,TNBC,和BC线模型。这是评估良性乳腺细胞以及原代和永生化BC细胞的EV的物理化学组成和蛋白质组的第一个可行性研究。我们的初步结果有望对BC的精准医学产生可能的影响。
Extracellular vesicles (
EVs) shuttle proteins, RNA, DNA, and lipids crucial for cell-to-cell communication. Recent findings have highlighted that
EVs, by virtue of their cargo, may also contribute to breast cancer (BC) growth and metastatic dissemination. Indeed,
EVs are gaining great interest as non-invasive cancer biomarkers. However, little is known about the biological and physical properties of EVs from malignant BC lesions, and even less is understood about EVs from non-malignant lesions, such as breast fibroadenoma (FAD), which are clinically managed using conservative approaches. Thus, for this pilot
study, we attempted to purify and explore the proteomic profiles of
EVs from benign breast lesions, HER2+ BCs, triple-negative BCs (TNBCs), and continuous BC cell lines (i.e., BT-549, MCF-10A, and MDA-MB-231), combining experimental and semi-quantitative approaches. Of note, proteome-wide analyses showed 49 common proteins across EVs harvested from FAD, HER2+ BCs, TNBCs, and model BC lines. This is the first feasibility
study evaluating the physicochemical composition and proteome of
EVs from benign breast cells and primary and immortalized BC cells. Our preliminary results hold promise for possible implications in precision medicine for BC.