Abstract：
Glial neoplasms are a group of diseases with poor prognoses. Not all risk factors are known, and no screening tests are available. Only histology provides certain diagnosis. As already reported, DNA transported by exosomes can be an excellent source of information shared by cells locally or systemically. These vesicles seem to be one of the main mechanisms of tumor remote intercellular signaling used to induce immune deregulation, apoptosis, and both phenotypic and genotypic modifications. In this study, we evaluated the exosomal DNA (exoDNA) concentration in blood samples of patients affected by cerebral glioma and correlated it with histological and radiological characteristics of tumors. From 14 patients with diagnosed primary or recurrent glioma, we obtained MRI imaging data, histological data, and preoperative blood samples that were used to extract circulating exosomal DNA, which we then quantified. Our results demonstrate a relationship between the amount of circulating exosomal DNA and tumor volume, and mitotic activity. In particular, a high concentration of exoDNA was noted in low-grade gliomas. Our results suggest a possible role of exoDNAs in the diagnosis of brain glioma. They could be particularly useful in detecting early recurrent high-grade gliomas and asymptomatic low-grade gliomas.
摘要：
胶质肿瘤是一组预后不良的疾病。并非所有的危险因素都是已知的，并且没有可用的筛查测试。只有组织学才能提供一定的诊断。正如已经报道的那样，外泌体转运的DNA可以是细胞局部或全身共享的极好的信息来源。这些囊泡似乎是肿瘤远程细胞间信号用于诱导免疫失调的主要机制之一，凋亡，以及表型和基因型修饰。在这项研究中,我们评估了受脑胶质瘤影响的患者血液样本中的外泌体DNA（exoDNA）浓度，并将其与肿瘤的组织学和放射学特征相关联。从14例诊断为原发性或复发性胶质瘤的患者中，我们获得了核磁共振成像数据,组织学数据,术前用于提取循环外泌体DNA的血液样本，然后我们量化了。我们的结果表明循环外泌体DNA的量和肿瘤体积之间的关系，和有丝分裂活性。特别是,在低级别神经胶质瘤中发现了高浓度的exoDNA.我们的结果表明，exoDNA在脑胶质瘤的诊断中可能具有作用。它们在检测早期复发的高级别神经胶质瘤和无症状的低级别神经胶质瘤中特别有用。
