Sludge is an inevitable waste product of sewage treatment with a high water content and large volume, it poses a significant threat of secondary pollution to both water and the atmosphere without proper disposal. In this regard, dewatering has emerged as an attractive method in sludge treatment, as it can reduce the sludge volume, enhance its transportability and calorific value, and even decrease the production of landfill leachate. In recent years, physical conditioning methods including non-chemical conditioners or energy input alone, have been extensively researched for their potential to enhance sludge dewatering efficiency, such as thermal treatment, freeze-thaw, microwave, ultrasonic, skeleton builders addition, and electro-dewatering, as well as combined methods. The main objective of this paper is to comprehensively evaluate the dewatering capacity of various physical conditioning methods, and identify key factors affecting sludge dewatering efficiency. In addition, future research anticipated directions and outlooks are proposed. This work is expected to provide valuable insights for developing efficient, eco-friendly, and low-energy consumption techniques for deep sludge dewatering.

Cyanobacteria are ideally suited for developing sustainable biological products but are underdeveloped due to a lack of genetic tools. Exopolysaccharide (EPS) is one of the essential bioproducts with widespread industrial applications. Despite their unique structural characteristics associated with distinct biological and physicochemical aspects, EPS from cyanobacteria has been underexplored. However, it is expected to accelerate in the near future due to the utilization of low-cost cyanobacterial platforms and readily available information on the structural data and specific features of these biopolymers. In recent years, cyanobacterial EPSs have attracted growing scientific attention due to their simple renewability, rheological characteristics, massive production, and potential uses in several biotechnology domains. This review focuses on the most recent research on potential new EPS producers and their distinct compositions responsible for novel biological activities. Additionally, nutritional and process parameters discovered recently for enhancing EPS production and engineering strategies applied currently to control the biosynthetic pathway for enhanced EPS production are critically highlighted. The process intensification of previously developed EPS extraction and purification processes from cyanobacterial biomass is also extensively explained. Furthermore, the newly reported biotechnological applications of cyanobacterial exopolysaccharides are also discussed.

Emerging pollutants (EPs) emerged as a group of new compounds whose presence in the environment has been widely detected in Mexico. In this country, different concentrations of pharmaceutical compounds, pesticides, dyes, and microplastics have been reported, which vary depending on the region and the analyzed matrix (i.e., wastewater, surface water, groundwater). The evidence of the EPs\' presence focuses on the detection of them, but there is a gap in information regarding is biomonitoring and their effects in health in Mexico. The presence of these pollutants in the country associated with lack of proper regulations in the discharge and disposal of EPs. Therefore, this review aims to provide a comprehensive view of the current environmental status, policies, and frameworks regarding Mexico\'s situation. The review also highlights the lack of information about biomonitoring since EPs are present in water even after their treatment, leading to a critical situation, which is high exposure to humans and animals. Although, technologies to efficiently eliminate EPs are available, their application has been reported only at a laboratory scale thus far. Here, an overview of health and environmental impacts and a summary of the research works reported in Mexico from 2014 to 2023 were presented. This review concludes with a concrete point of view and perspective on the status of the EPs\' research in Mexico as an alert for government entities about the necessity of measures to control the EPs disposal and treatment.

Anaerobic ammonium oxidation (anammox) granular sludge is a promising biotechnological process for treating low-carbon nitrogenous wastewater, and is featured with low energy consumption and footprint. Previous theoretical and experimental research on anammox granular sludge processes mainly focused on granulation (flocs → granules), but pay little attention to the granulation cycle including granulation and regeneration. This work reviewed the previous studies from the perspective of anammox granules lifecycle and proposed various sustainable formation mechanisms of anammox granules. By reviewing the anaerobic, aerobic, and anammox granulation mechanisms, we summarize the mechanisms of thermodynamic theory, heterogeneous growth, extracellular polymeric substance (EPS)-based adhesion, quorum sensing (QS)-based regulation, biomineralization-based growth, and stratification of microorganisms to understand anammox granulation. In the regeneration process, the formation of precursors for re-granulation is explained by the mechanisms of physical crushing, quorum quenching and dispersion cue sensing. Based on the granulation cycle mechanism, the rebuilding of the normal regeneration process is considered essential to avoid granule floatation and the wash-out of granules. This comprehensive review indicates that future research on anammox granulation cycle should focus on the effects of filamentous bacteria in denitrification-anammox granulation cycle, the role of QS/ quorum quenching (QQ)-based autoinducers, development of diversified mechanisms to understand the cycle and the cycle mechanisms of stored granules.

While exercise benefits a wide spectrum of diseases and affects most tissues and organs, many aspects of its underlying mechanistic effects remain unsolved. In vitro exercise, mimicking neuronal signals leading to muscle contraction in vitro, can be a valuable tool to address this issue. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines for this systematic review and meta-analysis, we searched EMBASE and PubMed (from database inception to 4 February 2022) for relevant studies assessing in vitro exercise using electrical pulse stimulation to mimic exercise. Meta-analyses of mean differences and meta-regression analyses were conducted. Of 985 reports identified, 41 were eligible for analysis. We observed variability among existing protocols of in vitro exercise and heterogeneity among protocols of the same type of exercise. Our analyses showed that AMPK, Akt, IL-6, and PGC1a levels and glucose uptake increased in stimulated compared to non-stimulated cells, following the patterns of in vivo exercise, and that these effects correlated with the duration of stimulation. We conclude that in vitro exercise follows motifs of exercise in humans, allowing biological parameters, such as the aforementioned, to be valuable tools in defining the types of in vitro exercise. It might be useful in transferring obtained knowledge to human research.

Bacteria are capable of colonizing industrial processing surfaces creating biofilms on them which may adversely affect the quality and safety of products. Traditional cleaning-in-place (CIP) treatments using caustic and nitric acid solutions have been known to exhibit variable efficiency in eliminating biofilm bacteria. Here, we introduce enzymes as an alternative to traditional CIP treatments and discuss their mechanism of action against bacterial biofilms in cheese manufacturing. In addition, we discuss research gaps namely thermal stability, substrate specificity and residual activity of enzymes that may play a vital role in the selection of enzymes with optimal effectiveness against multi species biofilms. The outcome of this mini review will aid in the development of a novel and sustainable enzyme-based CIP treatment during cheese manufacturing in the future.

Among the several biofilm-based bioreactors, moving bed biofilm reactors (MBBR) have been extensively used for wastewater treatment due to low operational costs, technical feasibility, and stability. Biofilm forming strains, e.g., Stenotrophomonas maltophila DQ01, achieved 94.21% simultaneous nitrification and denitrification (SND) and 94.43% removal of total nitrogen (TN) at a cycle time of 7 h, and a biofilm consortium consisting of Chryseobacteriumsp. andRhodobactersp. achieved 86.8% removal of total organic carbon (TOC) at hydraulic retention time (HRT) of 24 h using lab-scale MBBR. Modifications in the surface properties of the biocarrier materials achieved 99.5 ± 1.1% chemical oxygen demand (COD) and 93.6 ± 2.3% NH4+-N removal, significantly higher than the conventional commercial carrier. This review article summarizes the application of MBBR technology for wastewater treatment. The importance of bacterial biofilm and extracellular polymeric substances (EPS), anammox-n-DAMO coupled processes, and carrier surface modifications in MBBR technology have also been discussed.

This mini-review reports the effect of aerobic granular sludge (AGS) on performance and membrane-fouling in combined aerobic granular sludge-membrane bioreactor (AGS-MBR) systems. Membrane-fouling represents a major drawback hampering the wider application of membrane bioreactor (MBR) technology. Fouling can be mitigated by applying aerobic granular sludge technology, a novel kind of biofilm technology characterized by high settleability, strong microbial structure, high resilience to toxic/recalcitrant compounds of industrial wastewater, and the possibility to simultaneously remove organic matter and nutrients. Different schemes can be foreseen for the AGS-MBR process. However, an updated literature review reveals that in the AGS-MBR process, granule breakage represents a critical problem in all configurations, which often causes an increase of pore-blocking. Therefore, to date, the objective of research in this sector has been to develop a stable AGS-MBR through multiple operational strategies, including the cultivation of AGS directly in an AGS-MBR reactor, the occurrence of an anaerobic-feast/aerobic-famine regime in continuous-flow reactors, maintenance of average granule dimensions far from critical values, and proper management of AGS scouring, which has been recently recognized as a crucial factor in membrane-fouling mitigation.

Microbial extracellular polymeric substances (EPS) have a profound role in various wastewater treatment and reclamation processes, in which a variety of technologies are used for disinfection and microbial growth inhibition. These treatment processes can induce significant changes in the quantity and properties of EPS, and altered EPS could further adversely affect the wastewater treatment and reclamation system, including membrane filtration, disinfection, and water distribution. To clarify the effects of microbial inactivation approaches on EPS, these effects were classified into four categories: (1) chemical reactions, (2) cell lysis, (3) changing EPS-producing metabolic processes, and (4) altering microbial community. Across these different effects, treatments with free chlorine, methylisothiazolone, TiO2, and UV irradiation typically enhance EPS production. Among the residual microorganisms in EPS matrices after various microbial inactivation treatments, one of the most prominent is Mycobacterium. With respect to EPS properties, proteins and humic acids in EPS are usually more susceptible to treatment processes than polysaccharides. The affected EPS properties include changes in molecular weight, hydrophobicity, and adhesion ability. All of these changes can undermine wastewater treatment and reclamation processes. Therefore, effects on EPS quantity and properties should be considered during the application of microbial inactivation and growth inhibition techniques.

Sludge is an inevitable by product of sewage treatment, and it includes pathogens, heavy metals, organic pollutants and other toxic substances. The components of sludge are complex and variable with extracellular polymeric substances (EPS) being one. EPS are highly hydrophilic and compressible, and make sludge dewatering difficult. Therefore, the development of efficient sludge-dewatering technology is an important means of mitigating rapid sludge growth. At present, the main methods used for sludge deep-dewatering technology are chemical preconditioning with high-pressure filtration and electrical mechanical dewatering. The selection of chemical preconditioning directly determines the final efficiency of the sludge-dewatering process. In this paper, we conduct a comprehensive review of the problems related to sludge dewatering and systematically summarise the impact of different chemical conditioning technologies on the efficiency of sludge dewatering. Furthermore, the characteristics of different enhanced dewatering technologies are evaluated and analysed for their adaptability and final disposal methods. We believe that this review can clarify the chemical conditioner mechanism to improve sludge dewatering, provide reference debugging information for the sludge-dewatering process and promote the development of efficient and environmentally friendly sludge-dewatering technology.