目的:我们评估了补充omega(n)-3脂肪酸和饮食鱼摄入对成人炎症的全身脂质介质的联合影响。
方法:在VITAL中,一项双盲随机对照试验,成人随机接受ω-3脂肪酸(460mgEPA+380mgDHA/d)或安慰剂治疗.我们选择了报告低(<1份/月)基线饮食鱼摄入量的参与者,并按年龄与他们相匹配。性别,种族,并对自我报告鱼类摄入量最高(≥3.9份/周)的参与者进行试验。测试基线和1-y血浆样品的9种ω-3脂肪酸衍生的脂质介质。多变量线性模型评估了脂质介质的变化以及ω-3脂肪酸补充和膳食鱼类摄入量的联合影响。
结果:48名基线鱼类摄入量低的参与者与48名鱼类摄入量高的参与者相匹配。平均年龄为64.6(±7.26),50%是女性,85%的非西班牙裔白人.与安慰剂相比,在接受ω-3脂肪酸的患者中观察到预期方向的一年脂质介质变化:促炎介质减少,PGD2,5-HETE,和12-HETE;促分辨介体的增加,EPA和DHA。较大的1-y脂质生物标志物变化在那些低基线鱼摄入量随机服用DHA活性ω-3脂肪酸,EPA,观察到PGD2,ResolvinD1和ResolvinD4,尽管没有检测到显著的乘法相互作用。
结论:所有参与者补充1-yω-3脂肪酸与安慰剂相比,发现循环促分解和促炎介质的有益变化,在基线鱼类摄入量低的人群中,有更大的影响趋势,虽然相互作用并不显著。
OBJECTIVE: We assessed the joint effects of omega (n)-3 fatty acid supplementation and dietary fish intake on systemic lipid mediators of inflammation among adults.
METHODS: Within VITAL, a double-blind randomized controlled
trial, adults were randomized to ω-3 fatty acids (460 mg EPA + 380 mg
DHA/d) or placebo. We selected participants who reported low (<1 serving/mo) baseline dietary fish intake and matched them by age, sex, race, and
trial arm to participants with self-reported highest fish intake (≥3.9 servings/wk). Baseline and 1-y plasma samples were tested for 9 ω-3 fatty acid-derived lipid mediators. Multivariable linear models assessed lipid mediator changes and joint effects of ω-3 fatty acid supplementation and dietary fish intake.
RESULTS: Forty-eight participants with low baseline fish intake were matched to 48 with high fish intake. Mean age was 64.6 (±7.26), 50% were female, and 85% non-Hispanic white. One-year lipid mediator changes in expected directions were observed in those receiving ω-3 fatty acids versus placebo: reductions in proinflammatory mediators, PGD2, 5-HETE, and 12-HETE; increases in proresolving mediators, EPA and
DHA. Larger 1-y lipid biomarker changes were seen in those with low baseline fish intake randomized to active ω-3 fatty acids for
DHA, EPA, PGD2, Resolvin D1, and Resolvin D4 were observed, although no significant multiplicative interactions were detected.
CONCLUSIONS: Beneficial changes in circulating proresolving and proinflammatory mediators were found with 1-y of ω-3 fatty acid supplementation versus placebo for all participants, with a trend toward larger effects among those with low baseline fish intake, although interactions were not significant.