妊娠滋养细胞疾病(GTDs)是一组异质性病变,最常见的是葡萄胎(HM)。HMs通常在手术治疗后或在持续滋养细胞活动的情况下在化疗后治愈。作为一线或二线治疗,免疫治疗可能是一种有趣的替代方案。然而,只有少数研究探索了HMs的免疫微环境。在目前的回顾性研究中,包括19个完全痣和17个部分痣,我们使用以下抗体通过免疫组织化学检查免疫细胞微环境的组成:CD4,CD8,CD56,PD-L1,S100,CD83,CD207,CD123,CD1a,CD11c,CD163、PAX5和MUM1。在蜕膜细胞室中,以CD11c+细胞为主,其次是CD4+细胞,CD56+NK细胞,CD163+巨噬细胞,和CD8+T淋巴细胞。在子宫内膜腺室,以CD11c+细胞为主,其次是CD4+细胞,CD56+NK细胞,和CD8+T淋巴细胞。在绒毛隔间里,主要的免疫细胞是CD4+细胞,其次是CD163+巨噬细胞和CD11c+细胞。在所有三个隔室中的部分和完全痣之间观察到统计学上的显著差异。HMs的免疫微环境是免疫抑制的,但是完全和部分痣不同,后者具有较高的细胞浸润,表型提示免疫抑制活性。
Gestational trophoblastic diseases (GTDs) are a heterogeneous group of lesions, the most frequent being the hydatidiform mole (HM). HMs are usually cured after surgical treatment or after chemotherapy in the case of a persistent trophoblastic activity. Immunotherapy could be an interesting alternative as a first-line or second-line treatment. However, only a few studies have explored the immune microenvironment of HMs. In the present retrospective study including 19 complete and 17 partial moles, we examined the composition of the immune cell microenvironment by immunohistochemistry using the following antibodies: CD4, CD8, CD56, PD-L1, S100, CD83, CD207, CD123, CD1a, CD11c, CD163, PAX5, and MUM1. In the decidual cells compartment, CD11c+ cells were the predominant population, followed by CD4+ cells, CD56+ NK cells, CD163+ macrophages, and CD8+ T lymphocytes.In the endometrial glands compartment, CD11c+ cells were the predominant population, followed by CD4+ cells, CD56+ NK cells, and CD8+ T lymphocytes. In the villi compartment, the predominant immune cells were CD4+ cells, followed by CD163+ macrophages and CD11c+ cells. Statistically significant differences were observed between partial and complete moles in all three compartments. The immune microenvironment of HMs is immunosuppressive, but it differs between complete and partial moles, the latter having a higher infiltrate of cells with phenotypes suggestive of immunosuppressive activities.