栽培葡萄最严重的问题是病原微生物,这降低了水果的产量和质量。葡萄最普遍的疾病之一是“灰霉病”,由灰霉病菌引起的.一些芽孢杆菌菌株,如盐芽孢杆菌,解淀粉芽孢杆菌,和Velezensis芽孢杆菌,已知对主要的收获后植物腐烂具有活性。在这项研究中,我们表明,内生细菌B.velezensis菌株AMR25从野生葡萄葡萄葡萄的叶子中分离出来。对葡萄病原体表现出抗菌活性,包括B.cinerea。维氏芽孢杆菌AMR25的基因组具有一条长度为3,909,646bp的环状染色体。3689个开放阅读框架。基因组分析确定了参与聚酮化合物非核糖体合成的十个基因簇(大乳素,杆菌,和difficidin),脂肽(表面活性素,芬霉素,和bacillizin),和细菌素(艰难肽)。此外,研究中的基因组包含许多参与根定植的基因,生物膜的形成,和植物激素的生物合成。因此,内生细菌维氏芽孢杆菌菌株AMR25在开发创新的生物制品以增强植物对各种病原体的抗性方面显示出巨大的希望。
The most serious problems for cultivated grapes are pathogenic microorganisms, which reduce the yield and quality of fruit. One of the most widespread disease of grapes is \"gray mold\", caused by the fungus Botrytis cinerea. Some strains of Bacillus, such as Bacillus halotolerans, Bacillus amyloliquefaciens, and Bacillus velezensis, are known to be active against major post-harvest plant rots. In this study, we showed that the endophytic bacteria B. velezensis strain AMR25 isolated from the leaves of wild grapes Vitis amurensis Rupr. exhibited antimicrobial activity against grape pathogens, including B. cinerea. The genome of B. velezensis AMR25 has one circular chromosome with a length of 3,909,646 bp. with 3689 open reading frames. Genomic analysis identified ten gene clusters involved in the nonribosomal synthesis of polyketides (macrolactin, bacillene, and difficidin), lipopeptides (surfactin, fengycin, and bacillizin), and bacteriocins (difficidin). Also, the genome under study contains a number of genes involved in root colonization, biofilm formation, and biosynthesis of phytohormones. Thus, the endophytic bacteria B. velezensis strain AMR25 shows great promise in developing innovative biological products for enhancing plant resistance against various pathogens.