Animal remains are a common find in prehistoric and protohistoric funerary contexts. While taphonomic and osteological data provide insights about the proximate (depositional) factors responsible for these findings, the ultimate cultural causes leading to this observed mortuary behavior are obscured by the opacity of the archaeological record and the lack of written sources. Here, we apply an interdisciplinary suite of analytical approaches (zooarchaeological, anthropological, archaeological, paleogenetic, and isotopic) to explore the funerary deposition of animal remains and the nature of joint human-animal burials at Seminario Vescovile (Verona, Northern Italy 3rd-1st c. BCE). This context, culturally attributed to the Cenomane culture, features 161 inhumations, of which only 16 included animal remains in the form of full skeletons, isolated skeletal parts, or food offerings. Of these, four are of particular interest as they contain either horses (Equus caballus) or dogs (Canis lupus familiaris)-animals that did not play a dietary role. Analyses show no demographic, dietary, funerary similarities, or genetic relatedness between individuals buried with animals. Isotopic data from two analyzed dogs suggest differing management strategies for these animals, possibly linked to economic and/or ritual factors. Overall, our results point to the unsuitability of simple, straightforward explanations for the observed funerary variability. At the same time, they connect the evidence from Seminario Vescovile with documented Transalpine cultural traditions possibly influenced by local and Roman customs.

OBJECTIVE: To explore the relationship between the volumetric bone mineral density (vBMD), the thickness of the condylar cortex (Tcortex) and the hemimandibular volumes (Vhemimandible) of symmetrical and asymmetrical mandibles of children.
METHODS: The data collection consisted of 92 archeological skeletal remains of children\'s mandibles between 1 and 12 years old. The mandibles were digitalized with a computed tomography (CT) scan, and three dimensional models were obtained. Vhemimandible was calculated using the optimal symmetry plane. The volumes were used to calculate the asymmetry index (AI). Mandibles with an AI of ≥ 3% (N = 9) and a sample of the most symmetrical mandibles (N = 9) were selected for this research. Three groups were created: a symmetrical, an asymmetrical and a pooled group. Micro-CT was used to measure the vBMD and Tcortex in four volumes of interest. The AI was calculated for these parameters as well.
RESULTS: Significant correlations were found between the vBMD and the Tcortex in the pooled group (P < .01) and between the AI of the vBMD and the AI of the Tcortex in the pooled (P < .01) and symmetrical group (P < .05). No significant correlations were found between the vBMD and the Vhemimandible and between the respective AIs. Between the Tcortex and the Vhemimandible a significant correlation was found in the pooled and asymmetrical group.
CONCLUSIONS: There is a relationship between the vBMD and the Tcortex. The correlations between the Tcortex and the Vhemimandible are insufficient to draw firm conclusions. A relationship between the vBMD and Vhemimandible was not confirmed in this study.

In forensic anthropology, the estimation of skeletal sex results in a dichotomous outcome: male or female. This poses challenges when dealing with transgender individuals. Italy has the highest rate per capita of transgender murders, making their identification a compelling issue. This study presents three cases of unidentified skeletal remains of transgender women (MtF) that underwent autopsies in the 1990s at the Institute of Legal Medicine of Milan. The transgender status of the individuals was determined from the autopsy records which indicated the co-existence of breast implants and male genitalia. The biological profile was created by estimating sex, age, ancestry, and stature, and the bones were investigated looking for evidence of Facial Feminization Surgery (FFS). Anthropological estimations revealed that the skeletons belong to male, adults, European individuals, between 164 and 184 cm. Fragmented gelatinous masses were found in association with two skeletons, which were interpreted as remnants of breast implants. In two cases, signs of remodeling of the cortical surface were observed on the zygomatic bones, although the bone marks observed here were not specific enough to link them to FFS. Despite some limitations, this study highlights the need for greater awareness among practitioners about this limitedly addressed issue, advocating for a more inclusive forensic anthropology that strives to improve methods and interpretation of evidence for the identification of transgender individuals.

UNASSIGNED: Identification of humans has medicolegal relevance in civil issues involving property inheritance, marriage, contested sex, admission to educational institutions, and the disappearance of individuals. To determine the sex of human remains, forensic anthropologists usually use morphologic and metric approaches. This study was conducted to determine stature and sex according to mandibular dimensions in postmortem autopsy samples.
UNASSIGNED: A total of 150 mandibles from people 18-65 years of age were studied. Stature and sex were noted, and six mandibular measurements were taken with Vernier calipers, flexible measuring tape, a protractor, and a graduated autopsy table. Statistical analysis of the measured parameters was conducted in SPSS software. Statistical parameters, such as mean, standard deviation, Spearman\'s correlation coefficient, multiple linear regression, stepwise regression, and Mann-Whitney U test were analyzed.
UNASSIGNED: Bicondylar width was the stature estimation predictor with the strongest correlation (r = 0.439). The other parameters significantly associated with stature were bigonial width (p = 0.000), mandibular angle (p = 0.004), and mandibular arch length (p = 0.000), according to Spearman\'s correlation coefficient. The mandibular angle had the greatest dimorphic statistical significance (p = 0.004) according to the Mann-Whitney U test.
UNASSIGNED: Bicondylar width may serve as a valuable tool for estimating stature, and mandibular angle can be used to identify sex. Our findings may help forensic anthropologists estimate stature and identify human remains.
UNASSIGNED: في القضايا المدنية التي تتضمن ارث العقارات، الزواج، الجنس المتنازع عليه، القبول في المؤسسات التعليمية، واختفاء الأفراد، يكون التعرف على الهوية ذا أهمية قانونية طبية. لتحديد جنس البقايا البشرية، يستخدم عادة العلماء الجنائيون النهج المورفولوجي والمتري. لذلك، تم إجراء هذه الدراسة لتحديد القامة والجنس من أبعاد الفك السفلي بين العينات ما بعد الوفاة التي تم فحصها بواسطة التشريح.
UNASSIGNED: تمت دراسة 150 فك تتراوح أعمارها بين 18 - 65 سنة. تم تسجيل القامة والجنس وتم أخذ 6 قياسات للفك السفلي باستخدام المعيار فيرنيير، شريط القياس المرن، الدوائر المنسدلة، وجدول التشريح المتدرج. تم تحليل القياسات المسجلة إحصائيا. تم تحليل المؤشرات الإحصائية مثل المتوسط، الانحرافات المعيارية، معامل ارتباط سبيرمان، الانحدار الخطي المتعدد، انحدار الخطوة واختبار مان-ويتني.
UNASSIGNED: وجدت الدراسة أن عرض الفك السفلي يعد واحدا من المؤشرات لتقدير القامة مع أقوى ارتباط. القياسات الأخرى التي أظهرت ارتباطا كبيرا بالقامة كانت عرض الفك السفلي، وزاوية الفك، وطول قوس الفك باستخدام معامل ارتباط سبيرمان. عرضت زاوية الفك أكبر أهمية إحصائية ثنائية في تحليلنا في اختبار مان-ويتني.
UNASSIGNED: أبرزت هذه الدراسة أن عرض الفك السفلي يمكن أن يكون أداة قيمة لتقدير القامة. يمكن استخدام زاوية الفك لتحديد الجنس. لذا، ستساعد نتيجة دراستنا العلماء الجنائيين في تقدير القامة وتحديد البقايا البشرية.

Stable isotope methods for provenance of unidentified human remains are relatively a newer field of enquiry in forensic archeology. It is of great interest for forensic experts these days. The application of strontium isotope analyses for estimating geolocation of archeological remains is of great interest in bioarcheology and modern forensics. The strontium (Sr) isotope composition of human bones and teeth has been widely used to reconstruct an individual\'s geo-affiliation, residential mobility, and migration history. Thousands of unknown human remains, reportedly belonging to 282 Indian soldiers of 26th Native Bengal regiment and killed in 1857, were exhumed non-scientifically from an abandoned well situated underneath a religious structure at Ajnala (Amritsar, India). Whether these remains belonged to the individuals, local or non-local to the site, was the important forensic archeological question to be answered by doing their thorough forensic anthropological examinations. In the present study, 27 mandibular teeth (18 s molars, 6 first molars, and 3 premolars) collected from the Ajnala skeletal assemblage were processed for strontium isotope analysis, and the measured ratios were compared with published isotope baseline data to estimate the locality status of these remains. The Sr isotopic values were concentrated in the range of 0.7175 to 0.7270. The comparative analysis of isotopic ratios revealed that most individuals buried in the Ajnala well have 87Sr/86Sr values close to the river as well as groundwater of the Gangetic plain (less radiogenic 87Sr/86Sr ~ 0.716); most likely originated near Varanasi (Uttar Pradesh, India) region, whereas the individuals with higher 87Sr/86Sr ratios (~ 0.7200) probably resided in the West Bengal and Bihar areas where the river as well as groundwater of the Gangetic plain is relatively more radiogenic. Thus, the strontium isotope results reveal that the Ajnala individuals did not grow up or live in the Amritsar region during their childhood, and this observation complemented the previous forensic anthropological and molecular findings. There is very little Indian data on the bioavailable strontium, so the inferences from the present study estimating Sr isotope abundances are expected to provide baseline data for future forensic provenance studies that will contribute to the global efforts of mapping Sr isotope variations by the isotope community.

UNASSIGNED: The Teotihuacan civilisation was the largest one in ancient Mesoamerica. The Teotihuacan city was born in the north-eastern Basin of Mexico around the second century BC, reached its peak in the fourth century AD, and had cultural influence throughout Mesoamerica. At its peak, the size of the city reached more than 20 km2, and the total population is estimated to have increased from 100,000 to 200,000. However, knowledge of the genetic background of the Teotihuacan people is still limited.
UNASSIGNED: We aimed to determine the mitogenome sequences of the Teotihuacan human remains and compare the ancient and present Mesoamericans. In addition, we aimed to identify the food habits of ancient Teotihuacans.
UNASSIGNED: We determined the mitogenome sequences of human remains dated to 250-636 cal AD using target enrichment-coupled next generation sequencing. We also performed stable isotope analysis.
UNASSIGNED: We successfully obtained nearly full-length sequences newly unearthed from a civilian dwelling in the Teotihuacan site. Teotihuacan mitochondrial DNA was classified into the haplogroups in present and ancient Mesoamericans. In addition, Teotihuacan individuals had a diet dependent on C4 plants such as maize.
UNASSIGNED: Genetic diversity varied among the Teotihuacans.

Sex estimation is crucial in biological profiling of skeletal human remains. Methods used for sex estimation in adults are less effective for sub-adults due to varied cranium patterns during the growth period. Hence, this study aimed to develop a sex estimation model for Malaysian sub-adults using craniometric measurements obtained through multi-slice computed tomography (MSCT). A total of 521 cranial MSCT dataset of sub-adult Malaysians (279 males, 242 females; 0-20 years old) were collected. Mimics software version 21.0 (Materialise, Leuven, Belgium) was used to construct three-dimensional (3D) models. A plane-to-plane (PTP) protocol was utilised to measure 14 selected craniometric parameters. Discriminant function analysis (DFA) and binary logistic regression (BLR) were used to statistically analyze the data. In this study, low level of sexual dimorphism was observed in cranium below 6 years old. The level was then increased with age. For sample validation data, the accuracy of DFA and BLR in estimating sex improved with age from 61.6% to 90.3%. All age groups except 0-2 and 3-6 showed high accuracy percentage (≥75%) when tested using DFA and BLR. DFA and BLR can be utilised to estimate sex for Malaysian sub-adult using MSCT craniometric measurements. However, BLR showed higher accuracy than DFA in sex estimation of sub-adults.

Stable isotope analysis from bones and teeth has been widely used to estimate the likely geographic locations and dietary status of individuals whose osseous remains have been retrieved from some forensic or bio-archaeological contexts. The carbon and nitrogen stable isotope signatures can provide insights into geographic affinity and dietary habits. Ajnala skeletal remains represent a serious crime against humanity committed in past by colonial rulers and by amateur archaeologists of modern times. In present study, isotopic concentrations of carbon (δ13C) and nitrogen (δ15N) estimated from 21 mandibular molars have been used to estimate the local or non-local status of badly damaged skeletal remains retrieved from an abandoned well at Ajnala (India). The collagen samples having C/N ratio within range of 2.8-3.6 were considered as well-preserved and non-contaminated ones. The isotope concentrations of carbon and nitrogen varied from -18.7‰ to -22.9‰ and +7.6‰ to +11.7‰, with an average of -20.49 ± 1.2‰ and +9.31 ± 1.1‰, respectively. The analysis of the obtained isotope values reflected the consumption of C3/C4 mixed diet by majority of the individuals, and such type of dietary habits are mainly restricted to the reported Indo-Gangatic plain of India to which slain soldiers reportedly belonged to. These observations corroborated the previous observations about the geographic affinity and dietary status of Ajnala individuals. Though C and N isotopes are by and large not the confirmed/direct indicators of geographic origin, they can provide corroboratory information to support other observations narrowing down the dietary habits of individuals of certain specific geographical regions.

In 2017, a series of human remains corresponding to the executed leaders of the \"January Uprising\" of 1863-1864 were uncovered at the Upper Castle of Vilnius (Lithuania). During the archeological excavations, 14 inhumation pits with the human remains of 21 individuals were found at the site. The subsequent identification process was carried out, including the analysis and cross-comparison of post-mortem data obtained in situ and in the lab with ante-mortem data obtained from historical archives. In parallel, three anthropologists with diverse backgrounds in craniofacial identification and two students without previous experience attempted to identify 11 of these 21 individuals using the craniofacial superimposition technique. To do this, the five participants had access to 18 3D scanned skulls and 14 photographs of 11 different candidates. The participants faced a cross-comparison problem involving 252 skull-face overlay scenarios. The methodology follows the main agreements of the European project MEPROCS and uses the software Skeleton-ID™. Based on MEPROCS standard, a final decision was provided within a scale, assigning a value in terms of strong, moderate, or limited support to the claim that the skull and the facial image belonged (or not) to the same person for each case. The problem of binary classification, positive/negative, with an identification rate for each participant was revealed. The results obtained in this study make the authors think that both the quality of the materials used and the previous experience of the analyst play a fundamental role when reaching conclusions using the CFS technique.

The successful application of forensic genetic genealogy (FGG) to identify Jane and John Doe cases in the United States has raised the prospect of using the technique in Australia to assist in the reconciliation of unidentified human remains (UHRs) with long term missing persons. A study was conducted to explore the feasibility of FGG using whole genome array (WGA) data from both pristine control samples as well as compromised casework samples, with the view to explore how DNA quantity and quality impacted on the ability to generate search results when compared to a genetic genealogy database, such as GEDmatch. From this study, several insights were gained as to the impact DNA quantity and degradation had on the percentage of SNPs genotyped and heterozygote/homozygote ratio - which are critical for successful matching outcomes. It was noted in this study (using a control sample) that successful matching occurred when genotyping errors were 5% or less. Two UHR cases were matched to kits on GEDmatch PRO, which provided investigative leads for identification purposes. The effectiveness of the FGG approach to match casework samples (as well as volunteer samples used in the study) is indicative of the usage of \'direct-to-consumer\' (DTC) genetic testing by Australians. Given the (often) limited availability of casework samples, findings from this study will assist Australian agencies considering the use of FGG, to determine if WGA is a suitable method for their application.