肠产毒性脆弱拟杆菌(ETBF)已收到显著的关注,可能与,或因果作用,结直肠癌(CRC)。本综述的目的是评估已发表的支持(i)ETBF与CRC之间的关联以及(ii)ETBF在CRC中的因果作用的证据的状态。PubMed和Scopus搜索于2021年8月进行,以识别人类,动物,和细胞研究有关ETBF在CRC中的作用。纳入标准包括使用细胞系,老鼠,暴露于BFT或ETBF,和检测bft。综述研究被排除在外,研究仅限于英语。对细胞进行研究设计质量和偏倚风险分析,动物,以及使用ToxRTools的人体研究,SYRCLE,和NOS,分别。确定了95项符合条件的研究,这包括22项人体研究,24动物研究,43个细胞研究,和6项研究,包括细胞和小鼠研究。我们发现,绝大多数研究支持ETBF在CRC中的关联或因果作用。在体外和体内研究中检测到高水平的研究偏倚。研究设计和报告的高度异质性使得很难将这些发现综合成一个统一的结论。这表明需要未来的研究,包括改进的机械模型,纵向的体外和体内证据,并且需要对混杂因素进行适当控制,以确认ETBF是否在CRC病因发病机制中具有直接作用.
Enterotoxigenic Bacteroides fragilis (ETBF) has received significant attention for a possible association with, or causal role in, colorectal cancer (CRC). The goal of this
review was to assess the status of the published evidence supporting (i) the association between ETBF and CRC and (ii) the causal role of ETBF in CRC. PubMed and Scopus searches were performed in August 2021 to identify human, animal, and cell studies pertaining to the role of ETBF in CRC. Inclusion criteria included the use of cell lines, mice, exposure to BFT or ETBF, and detection of bft.
Review studies were excluded, and studies were limited to the English language. Quality of study design and risk of bias analysis was performed on the cell, animal, and human studies using ToxRTools, SYRCLE, and NOS, respectively. Ninety-five eligible studies were identified, this included 22 human studies, 24 animal studies, 43 cell studies, and 6 studies that included both cells and mice studies. We found that a large majority of studies supported an association or causal role of ETBF in CRC, as well as high levels of study bias was detected in the in vitro and in vivo studies. The high-level heterogeneity in study design and reporting made it difficult to synthesize these findings into a unified conclusion, suggesting that the need for future studies that include improved mechanistic models, longitudinal in vitro and in vivo evidence, and appropriate control of confounding factors will be required to confirm whether ETBF has a direct role in CRC etiopathogenesis.